Purification of Rabbit Tumor Necrosis Factor

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 1985 Jan 28

PMID 3967764

Citations 17

Authors

S Abe

T Gatanaga

M Yamazaki

G Soma

D Mizuno

Affiliations

Soon will be listed here.

Abstract

Rabbit tumor necrosis factor (TNF) was purified and shown by SDS-PAGE to be a single protein of 18 kDa. TNF in 355 ml rabbit serum was precipitated with ammonium sulfate, and purified by repeated DEAE-Sephadex and Sephacryl S-200 chromatographies, and the final fractionation on Blue-Sepharose 6B. By this procedure its yield was 22% and its specific activity was 2.4 X 10(7) U/mg protein. The sequence of the N-terminal 20 amino acids was determined.

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