Investigation of the Effects of Rainbow Trout (Oncorhynchus Mykiss) Skin Mucus Against Indomethacin-induced Gastric Damage in Rats

Overview

Journal J Mol Histol

Specialty Biochemistry

Date 2024 Dec 14

PMID 39673571

Authors

Hilal Bayir

Seyda Tacer

Emir Enis Yurdgulu

Abdulmecit Albayrak

Erdem Toktay

Yasin Bayir

Affiliations

Soon will be listed here.

Abstract

A peptic ulcer is a stomach lesion. It is generally caused by malnutrition, the use of anti-inflammatory medications, and an imbalance between mucosal defense systems. In fishes, the lubricous substance that called mucus secreted from the skin, prevents the entry of microorganisms that can enter the body through the skin. That mucus contains immune components such as antimicrobial peptides, lysozymes, lectins, proteases, and mucin. This study investigated the antiulcer activity of Rainbow Trout skin Mucus (RTM) in indomethacin induced ulcer model of rats and compared with famotidine as standard antiulcer drug. We administered 50, 100 and 200 mg/kg RTM dose on indomethacin-induced gastric ulcer model in rats and evaluated the numerical density of ulcer areas, histopathologic parameters and oxidative stress parameters (SOD, GSH, MDA) in the rat stomach. RTM was able to inhibit indomethacin-induced ulcer formation and exhibited a similar effect to 40 mg/kg dose of standard drug famotidine. 200 mg/kg dose of RTM had positive effects on oxidative stress biomarkers and histopathological results in the stomach tissue of rats. This is the first time we have fully demonstrated the gastroprotective effects of RTM as a waste product in rats. Analyses have shown that mucin, which has a positive regulatory effect on oxidative stress parameters, may be responsible for the gastroprotective effect.

References

Ardalani H, Hadipanah A, Sahebkar A . Medicinal Plants in the Treatment of Peptic Ulcer Disease: A Review. Mini Rev Med Chem. 2019; 20(8):662-702. DOI: 10.2174/1389557520666191227151939. View

Bayir Y, Odabasoglu F, Cakir A, Aslan A, Suleyman H, Halici M . The inhibition of gastric mucosal lesion, oxidative stress and neutrophil-infiltration in rats by the lichen constituent diffractaic acid. Phytomedicine. 2006; 13(8):584-90. DOI: 10.1016/j.phymed.2005.07.002. View

Bi W, Man H, Man M . Efficacy and safety of herbal medicines in treating gastric ulcer: a review. World J Gastroenterol. 2014; 20(45):17020-8. PMC: 4258570. DOI: 10.3748/wjg.v20.i45.17020. View

Boushra A, Elsayed A, Ibrahim N, Abdelwahed M, Ahmed E . A comparative study on the possible protective effect of esomeprazole, spirulina, wheatgrass on indomethacin-induced gastric ulcer in male albino rats. Mol Biol Rep. 2019; 46(5):4843-4860. DOI: 10.1007/s11033-019-04933-1. View

Calik I, Yayla M, Cinar I, Cadirci E, Albayrak A, Sirin B . LP44 (4-[2-(methylthio)phenyl]-N-(1,2,3,4-tetrahydronaphthalen-1-yl)-1-piperazinehexanamide) exerts anti-ulcer effects via 5-hydroxytryptamine receptor 7 activation on indomethacin-induced gastric ulcers in rats. Inflammopharmacology. 2020; 28(4):893-902. DOI: 10.1007/s10787-020-00725-3. View

Dash S, Das S, Samal J, Thatoi H . Epidermal mucus, a major determinant in fish health: a review. Iran J Vet Res. 2018; 19(2):72-81. PMC: 6056142. View

Eisenstein E, Anstrom K, Kong D, Shaw L, Tuttle R, Mark D . Clopidogrel use and long-term clinical outcomes after drug-eluting stent implantation. JAMA. 2006; 297(2):159-68. DOI: 10.1001/jama.297.2.joc60179. View

El-Ashmawy N, Khedr E, El-Bahrawy H, Selim H . Nebivolol prevents indomethacin-induced gastric ulcer in rats. J Immunotoxicol. 2016; 13(4):580-9. DOI: 10.3109/1547691X.2016.1142488. View

Fallone C, Chiba N, Veldhuyzen Van Zanten S, Fischbach L, Gisbert J, Hunt R . The Toronto Consensus for the Treatment of Helicobacter pylori Infection in Adults. Gastroenterology. 2016; 151(1):51-69.e14. DOI: 10.1053/j.gastro.2016.04.006. View

10.

Feng J, Wang J, Wang Y, Huang X, Shao T, Deng X . Oxidative Stress and Lipid Peroxidation: Prospective Associations Between Ferroptosis and Delayed Wound Healing in Diabetic Ulcers. Front Cell Dev Biol. 2022; 10:898657. PMC: 9304626. DOI: 10.3389/fcell.2022.898657. View

11.

Garcia-Meilan I, Tort L, Khansari A . Rainbow trout integrated response after recovery from short-term acute hypoxia. Front Physiol. 2022; 13:1021927. PMC: 9634174. DOI: 10.3389/fphys.2022.1021927. View

12.

Johansson M, Sjovall H, Hansson G . The gastrointestinal mucus system in health and disease. Nat Rev Gastroenterol Hepatol. 2013; 10(6):352-61. PMC: 3758667. DOI: 10.1038/nrgastro.2013.35. View

13.

Kamisah Y, Qodriyah H, Chua K, Nur Azlina M . Vitamin E: a potential therapy for gastric mucosal injury. Pharm Biol. 2014; 52(12):1591-7. DOI: 10.3109/13880209.2014.902082. View

14.

Sezen Karaoglan E, Bayir Y, Albayrak A, Toktay E, Ozgen U, Kazaz C . Isolation of Major Compounds and Gastroprotective Activity of on Indomethacin Induced Gastric Ulcers in Rats. Eurasian J Med. 2020; 52(3):249-253. PMC: 7651754. DOI: 10.5152/eurasianjmed.2020.19243. View

15.

Laine L, Weinstein W . Histology of alcoholic hemorrhagic "gastritis": a prospective evaluation. Gastroenterology. 1988; 94(6):1254-62. DOI: 10.1016/0016-5085(88)90661-0. View

16.

Lanas A, Chan F . Peptic ulcer disease. Lancet. 2017; 390(10094):613-624. DOI: 10.1016/S0140-6736(16)32404-7. View

17.

Liu Y, Li B, Su Y, Zhao R, Song P, Li H . Potential activity of Traditional Chinese Medicine against Ulcerative colitis: A review. J Ethnopharmacol. 2022; 289:115084. DOI: 10.1016/j.jep.2022.115084. View

18.

Ohkawa H, Ohishi N, Yagi K . Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2):351-8. DOI: 10.1016/0003-2697(79)90738-3. View

19.

Polat B, Halici Z, Cadirci E, Albayrak A, Karakus E, Bayir Y . The effect of alpha-lipoic acid in ovariectomy and inflammation-mediated osteoporosis on the skeletal status of rat bone. Eur J Pharmacol. 2013; 718(1-3):469-74. DOI: 10.1016/j.ejphar.2013.07.033. View

20.

Repetto M, Llesuy S . Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz J Med Biol Res. 2002; 35(5):523-34. DOI: 10.1590/s0100-879x2002000500003. View