Dissemination Patterns and Functional Role of a Symbiotic Bacteria Stenotrophomonas Maltophilia in Phytoseiulus Persimilis

Overview

Journal Exp Appl Acarol

Specialties Biology
Parasitology

Date 2024 Dec 13

PMID 39671023

Authors

Hong Yan

Endong Wang

Xuenong Xu

Guo-Shu Wei

Bo Zhang

Affiliations

Soon will be listed here.

Abstract

Symbiotic bacteria play a crucial role in various facets of host biology and physiology. The development and utilization of symbiotic bacteria in insects show promising potential for enhancing their reproduction, temperature tolerance, resistances to pathogens and insecticides. However, limited research has been conducted on symbiotic bacteria in predatory mites. In Phytoseiulus persimilis, we successfully cultivated a strain of Stenotrophomonas maltophilia, which has been noted for its significant contributions to pathogen control, pesticide and toxin resistance, and nutrition provision in some insect species. To explore the effect of S. maltophilia and its potential application on predatory mites, we fed S. maltophilia to P. persimilis and evaluated the transmission dynamics within mite generations. We examined its impacts on predator fitness and resistances to pesticides, pathogens, and starvation. The results showed that the S. maltophilia content in the offspring increased by 12.91 times when gravid mites were fed with the bacterial solution. P. persimilis that consumed prey treated with S. maltophilia showed a 25.20-fold increase in microbial content. Mating with treated males did not affect microbial levels in females. Moreover, S. maltophilia did not cause any discernible effect on the fitness of P. persimilis, including survival, developmental duration, fecundity, and longevity. Notably, it was found to improve P. persimilis survival following exposure to the pathogen Acaricomes phytoseiuli, resulting in a reduction of mortality by 20% compared to the control. This study serves as a foundational step for further utilization of beneficial microbes to improve the efficacy of predatory mite biological control.

References

Bai S, Yao Z, Raza M, Cai Z, Zhang H . Regulatory mechanisms of microbial homeostasis in insect gut. Insect Sci. 2020; 28(2):286-301. DOI: 10.1111/1744-7917.12868. View

Becnel J, Jeyaprakash A, Hoy M, Shapiro A . Morphological and molecular characterization of a new microsporidian species from the predatory mite Metaseiulus occidentalis (Nesbitt) (Acari, Phytoseiidae). J Invertebr Pathol. 2002; 79(3):163-72. DOI: 10.1016/s0022-2011(02)00032-0. View

Bing X, Xia C, Ye Q, Gong X, Cui J, Peng C . Wolbachia manipulates reproduction of spider mites by influencing herbivore salivary proteins. Pest Manag Sci. 2022; 79(1):315-323. DOI: 10.1002/ps.7201. View

Ceja-Navarro J, Vega F, Karaoz U, Hao Z, Jenkins S, Lim H . Gut microbiota mediate caffeine detoxification in the primary insect pest of coffee. Nat Commun. 2015; 6:7618. PMC: 4510693. DOI: 10.1038/ncomms8618. View

Chojniak J, Jalowiecki L, Dorgeloh E, Hegedusova B, Ejhed H, Magner J . Application of the BIOLOG system for characterization of Serratia marcescens ss marcescens isolated from onsite wastewater technology (OSWT). Acta Biochim Pol. 2015; 62(4):799-805. DOI: 10.18388/abp.2015_1138. View

Deng S, Chen Y, Wang D, Shi T, Wu X, Ma X . Rapid biodegradation of organophosphorus pesticides by Stenotrophomonas sp. G1. J Hazard Mater. 2015; 297:17-24. DOI: 10.1016/j.jhazmat.2015.04.052. View

Douglas A . Multiorganismal insects: diversity and function of resident microorganisms. Annu Rev Entomol. 2014; 60:17-34. PMC: 4465791. DOI: 10.1146/annurev-ento-010814-020822. View

Fu R, Chen D, Tian G, Zheng P, Mao X, Yu J . Effect of dietary supplementation of or yeast hydrolysates on growth performance, antioxidant activity, cytokines and intestinal microflora of growing-finishing pigs. Anim Nutr. 2020; 5(4):366-372. PMC: 6920390. DOI: 10.1016/j.aninu.2019.06.003. View

Hayashi T, Hosokawa T, Meng X, Koga R, Fukatsu T . Female-specific specialization of a posterior end region of the midgut symbiotic organ in Plautia splendens and allied stinkbugs. Appl Environ Microbiol. 2015; 81(7):2603-11. PMC: 4357921. DOI: 10.1128/AEM.04057-14. View

10.

Hoy M, Jeyaprakash A . Symbionts, including pathogens, of the predatory mite Metaseiulus occidentalis: current and future analysis methods. Exp Appl Acarol. 2008; 46(1-4):329-47. DOI: 10.1007/s10493-008-9185-3. View

11.

Jeyaprakash A, Hoy M . Multiple displacement amplification in combination with high-fidelity PCR improves detection of bacteria from single females or eggs of Metaseiulus occidentalis (Nesbitt) (Acari: Phytoseiidae). J Invertebr Pathol. 2004; 86(3):111-6. DOI: 10.1016/j.jip.2004.05.005. View

12.

Kikuchi Y, Hayatsu M, Hosokawa T, Nagayama A, Tago K, Fukatsu T . Symbiont-mediated insecticide resistance. Proc Natl Acad Sci U S A. 2012; 109(22):8618-22. PMC: 3365206. DOI: 10.1073/pnas.1200231109. View

13.

Kucuk R . Gut Bacteria in the Holometabola: A Review of Obligate and Facultative Symbionts. J Insect Sci. 2020; 20(4). PMC: 7433766. DOI: 10.1093/jisesa/ieaa084. View

14.

Kwong W, Moran N . Gut microbial communities of social bees. Nat Rev Microbiol. 2016; 14(6):374-84. PMC: 5648345. DOI: 10.1038/nrmicro.2016.43. View

15.

Li H, Wan Q, Zhang S, Wang C, Su S, Pan B . Housefly larvae (Musca domestica) significantly accelerates degradation of monensin by altering the structure and abundance of the associated bacterial community. Ecotoxicol Environ Saf. 2018; 170:418-426. DOI: 10.1016/j.ecoenv.2018.12.013. View

16.

Luan J, Sun X, Fei Z, Douglas A . Maternal Inheritance of a Single Somatic Animal Cell Displayed by the Bacteriocyte in the Whitefly Bemisia tabaci. Curr Biol. 2018; 28(3):459-465.e3. PMC: 5807091. DOI: 10.1016/j.cub.2017.12.041. View

17.

Merlin B, Moraes G, Consoli F . The Microbiota of a Mite Prey-Predator System on Different Host Plants Are Characterized by Dysbiosis and Potential Functional Redundancy. Microb Ecol. 2022; 85(4):1590-1607. DOI: 10.1007/s00248-022-02032-6. View

18.

Moraes A, Videira S, Bittencourt V, Bittencourt A . Antifungal activity of Stenotrophomonas maltophilia in Stomoxys calcitrans larvae. Rev Bras Parasitol Vet. 2014; 23(2):194-9. DOI: 10.1590/s1984-29612014037. View

19.

Morales-Jimenez J, Zuniga G, Ramirez-Saad H, Hernandez-Rodriguez C . Gut-associated bacteria throughout the life cycle of the bark beetle Dendroctonus rhizophagus Thomas and Bright (Curculionidae: Scolytinae) and their cellulolytic activities. Microb Ecol. 2012; 64(1):268-78. DOI: 10.1007/s00248-011-9999-0. View

20.

Ozdal M, Ozdal O, Alguri O . Isolation and Characterization of α-Endosulfan Degrading Bacteria from the Microflora of Cockroaches. Pol J Microbiol. 2016; 65(1):63-8. DOI: 10.5604/17331331.1197325. View