SbcB Facilitates Natural Transformation in in an Exonuclease-independent Manner

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2024 Dec 13

PMID 39670763

Authors

Triana N Dalia

Ankur B Dalia

Affiliations

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Abstract

Importance: Horizontal gene transfer by natural transformation contributes to the spread of antibiotic resistance and virulence factors in bacterial species. Here, we study how one protein, SbcB, helps facilitate this process in the facultative bacterial pathogen . SbcB is a well-known for its exonuclease activity (i.e., the ability to degrade the ends of linear DNA). Through this study, we uncover that while SbcB is important for natural transformation, it does not facilitate this process using its exonuclease activity. Thus, this work helps further our understanding of the molecular events required for this conserved evolutionary process and uncovers a function for SbcB beyond its canonical exonuclease activity.

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