Protocol for Cellular RNA G-quadruplex Profiling Using G4RP.v2

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2024 Dec 11

PMID 39661503

Authors

Jeremie Mitteaux

David Monchaud

Affiliations

Soon will be listed here.

Abstract

The isolation of G-quadruplexes (G4s) from human cells using specific molecular tools constitutes an invaluable step forward in uncovering the biology of these higher-order DNA and RNA structures. Here, we present an improved version of the G4-RNA precipitation (G4RP) protocol developed to identify RNA G4s from human cancer cells. We describe steps for cell treatment and lysis, chemoprecipitation of G4s using TASQ tools, go/no-go steps, and quantitative reverse-transcription PCR (RT-qPCR) quantification and analysis. For complete details on the use and execution of this protocol, please refer to Mitteaux et al..

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