Protocol for Whole-mount Preparation, Clearing, and Visualization of the Adult Zebrafish Spinal Cord Structures

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2024 Dec 10

PMID 39656590

Authors

Mariana Rebocho da Costa

Ana Ribeiro

Raquel Quiteria

Dalila Neves-Silva

Leonor Saude

Affiliations

Soon will be listed here.

Abstract

The vascular repair, a pivotal element of the spinal cord (SC) injury response, has been notably neglected in zebrafish research. Here, we detail the extraction and dissection of whole-mount zebrafish SCs and the optimized whole-mount immunofluorescence staining and clearing protocols for the visualization of SC structures, such as the vascular network. Additional downstream applications of these samples, from gelatin embedding to cryosectioning and tissue staining, can be done after clearing reversion and are also described in this work. For complete details on the use and execution of this protocol, please refer to Ribeiro et al..

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