in Urine of Children with Monosymptomatic Nocturnal Enuresis

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2024 Dec 10

PMID 39654976

Authors

Naoto Nishizaki

Satoshi Oshiro

Mari Tohya

Shin Watanabe

Tadaharu Okazaki

Ken Takahashi

Teruo Kirikae

Toshiaki Shimizu

Affiliations

Soon will be listed here.

Abstract

Background: Despite a unique microbiome in urine, the relationship between nocturnal enuresis and the urobiome remains unclear. This study aimed to compare the presence of specific bacterial species in the urine of children with and without nocturnal enuresis.

Methods: We used 16S ribosomal RNA gene sequencing to analyze the urobiome in urine samples obtained from the two groups of children. The presence of was examined using real-time PCR in the urine of 25 children diagnosed with monosymptomatic nocturnal enuresis (MNE), and 17 children without this condition.

Results: Children with MNE exhibited a significantly higher prevalence of : 16 out of 25 (64.0%) compared to 4 out of 17 (23.5%) in the control group. Among children with frequent bedwetting, there was a significantly higher prevalence of ;15 out of 16 (93.8%) compared to 2 out of 9 (22.2%) in those with infrequent bedwetting. Bacterial culture tests confirmed the anaerobic growth of isolates from urine samples of two PCR-positive patients with MNE. These isolates were found to be susceptible to ampicillin.

Conclusion: These findings suggest that may be associated with chronic urinary tract infections and potentially contribute to the development of MNE in children.

Citing Articles

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Logan A, Mishra P, Prescott S Microb Biotechnol. 2025; 18(3):e70129.

PMID: 40072296 PMC: 11898878. DOI: 10.1111/1751-7915.70129.

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