Age-stratification Reveals Age-specific Intestinal Microbiota Signatures in Juvenile Idiopathic Arthritis

Overview

Journal Mol Cell Pediatr

Publisher Springer

Specialty Pediatrics

Date 2024 Dec 9

PMID 39653980

Authors

Lisa Budzinski

Toni Sempert

Leonie Lietz

Rene Maier

Gi-Ung Kang

Anne Sae Lim von Stuckrad

Carl Christoph Goetzke

Maria Roth

Aayushi Shah

Amro Abbas

Katrin Lehman

Kathleen Necke

Stefanie Bartsch

Ute Hoffmann

Mir-Farzin Mashreghi

Robert Biesen

Tilmann Kallinich

Hyun-Dong Chang

Affiliations

Soon will be listed here.

Abstract

Objective: Juvenile Idiopathic Arthritis (JIA) comprises diverse chronic inflammatory conditions driven by malfunction of the immune system. The intestinal microbiota is considered a crucial environmental factor correlating with chronic inflammatory diseases, and for JIA certain alterations in the microbiota have already been described.

Methods: Here, we have characterized intestinal microbiota samples from 54 JIA patients and 38 pediatric healthy controls by conventional 16S rRNA sequencing and by single-cell analysis for phenotypic features by multi-parameter microbiota flow cytometry (mMFC), which complements the population-based taxonomic profiling with the characterization of individual bacterial cells.

Results: We found age to be a crucial confounder in microbiota analyses of JIA patients. Age stratification revealed specific microbiota alterations neglected by the general comparison of JIA patients and pediatric controls.

Conclusion: Age groups presented distinct taxonomic profiles and microbiota phenotypic signatures which transitioned with age, highlighting changes in the microbiota-immune system interaction with age.

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