» Articles » PMID: 39651415

Using Chlorophyll Fluorescence Parameters and Antioxidant Enzyme Activity to Assess Drought Tolerance of Spring Wheat

Overview
Journal Photosynthetica
Date 2024 Dec 9
PMID 39651415
Authors
Affiliations
Soon will be listed here.
Abstract

The improvement of phenotyping methods is necessary for large-scale screening studies of wheat ( L.) drought tolerance. The objective of our research was to find out whether it is possible to use chlorophyll (Chl) fluorescence parameters instead of biochemical indicators of drought tolerance when screening wheat. We measured shoot biomass, gas exchange, as well as biochemical and Chl fluorescence indicators in 11 wheat genotypes grown under contrasting water supplies and differing in drought tolerance. The effect of drought on the traits was evaluated using the effect of size index. We made two independent rankings: one based on biochemical indicators and the other on Chl fluorescence parameters. The positions of the three genotypes with the highest comprehensive drought tolerance index in the two independent rankings coincided completely. It is concluded that Chl fluorescence methods are suitable for identifying soft wheat genotypes that differ significantly in their ability to activate cellular defense mechanisms.

References
1.
von Wettstein D . [Chlorophyll lethals & submicroscopic morphological changes in plastids]. Exp Cell Res. 1957; 12(3):427-506. DOI: 10.1016/0014-4827(57)90165-9. View

2.
de Lamotte F, Duviau M, Kobrehel K . Glutathione reductase in wheat grain. 1. Isolation and characterization. J Agric Food Chem. 2000; 48(10):4978-83. DOI: 10.1021/jf0003808. View

3.
Baier M, Noctor G, Foyer C, Dietz K . Antisense suppression of 2-cysteine peroxiredoxin in Arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism. Plant Physiol. 2000; 124(2):823-32. PMC: 59186. DOI: 10.1104/pp.124.2.823. View

4.
Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

5.
Varshney R, Tuberosa R, Tardieu F . Progress in understanding drought tolerance: from alleles to cropping systems. J Exp Bot. 2018; 69(13):3175-3179. PMC: 5991209. DOI: 10.1093/jxb/ery187. View