Dielectric Spectroscopy and TD-NMR Investigation for Assessing Water Solid Dynamics in Normal and Wooden Breast Chicken

Overview

Journal Poult Sci

Publisher Elsevier

Specialty Veterinary Medicine

Date 2024 Dec 7

PMID 39644718

Authors

Francesca Soglia

Eleonora Iaccheri

Luca Laghi

Luigi Ragni

Pedro Jose Fito Suner

Massimiliano Petracci

Affiliations

Soon will be listed here.

Abstract

Dielectric Spectroscopy (DS) and TimeDomain-Nuclear Magnetic Resonance (TD-NMR) were exploited to investigate water and solid dynamics in chicken's Pectoralis major muscles having macroscopically normal appearance (N) and affected by Wooden Breasts (WB) abnormality. 147 PMM were collected and classified as macroscopically normal (N) (N=74) or Wooden Breast (WB) (N=73) based on their visual appearance and manual palpation. Protons' T (transverse relaxation time), and dielectric properties were carried out. The dielectric constant ( [Formula: see text] and the loss factor ( [Formula: see text] of γ relaxation of WB samples was significantly higher than the one of N samples ( [Formula: see text] 59.8 and [Formula: see text] 18.3), while the WB revealed a low relaxation frequency (1.5 E+09) compared to N samples (1.7 E+09). WB showed a higher dielectric constant of relaxation and thus polarizability mainly due to free water, and lower relaxation frequency imputable to a more complex solid structure. This was confirmed by TD-NMR, evidencing a significant increase in the relative intensity of the proton population ascribable to the extra-myofibrillar water (32.9 vs. 21.5%; P<0.001) along with a reduction of the one occupying the intra-myofibrillar spaces (62.0 vs. 75.4%; P<0.001). These outcomes may be ascribed to the re-organization of the skeletal muscle structure associated with the onset and progression of the WB abnormality.

References

Schmidt S . Water and solids mobility in foods. Adv Food Nutr Res. 2004; 48:1-101. DOI: 10.1016/S1043-4526(04)48001-2. View

Sihvo H, Immonen K, Puolanne E . Myodegeneration with fibrosis and regeneration in the pectoralis major muscle of broilers. Vet Pathol. 2013; 51(3):619-23. DOI: 10.1177/0300985813497488. View

Huff-Lonergan E, Lonergan S . Mechanisms of water-holding capacity of meat: The role of postmortem biochemical and structural changes. Meat Sci. 2011; 71(1):194-204. DOI: 10.1016/j.meatsci.2005.04.022. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Morey A, Smith A, Garner L, Cox M . Application of Bioelectrical Impedance Analysis to Detect Broiler Breast Filets Affected With Woody Breast Myopathy. Front Physiol. 2020; 11:808. PMC: 7365903. DOI: 10.3389/fphys.2020.00808. View

Warner R, Kauffman R, Greaser M . Muscle protein changes post mortem in relation to pork quality traits. Meat Sci. 2011; 45(3):339-52. DOI: 10.1016/s0309-1740(96)00116-7. View

Nelson S . Fundamentals of dielectric properties measurements and agricultural applications. J Microw Power Electromagn Energy. 2011; 44(2):98-113. DOI: 10.1080/08327823.2010.11689778. View

Joo S, Kauffman R, Kim B, Park G . The relationship of sarcoplasmic and myofibrillar protein solubility to colour and water-holding capacity in porcine longissimus muscle. Meat Sci. 2011; 52(3):291-7. DOI: 10.1016/s0309-1740(99)00005-4. View

Pang B, Yu X, Bowker B, Zhang J, Yang Y, Zhuang H . Effect of meat temperature on moisture loss, water properties, and protein profiles of broiler pectoralis major with the woody breast condition. Poult Sci. 2021; 100(2):1283-1290. PMC: 7858045. DOI: 10.1016/j.psj.2020.10.034. View

10.

Zhang Y, Wang P, Xu X, Xia T, Li Z, Zhao T . Effect of wooden breast myopathy on water-holding capacity and rheological and gelling properties of chicken broiler breast batters. Poult Sci. 2020; 99(7):3742-3751. PMC: 7597808. DOI: 10.1016/j.psj.2020.03.032. View

11.

Traffano-Schiffo M, Castro-Giraldez M, Colom R, Fito P . Development of a Spectrophotometric System to Detect White Striping Physiopathy in Whole Chicken Carcasses. Sensors (Basel). 2017; 17(5). PMC: 5469629. DOI: 10.3390/s17051024. View

12.

Gabriel S, Lau R, Gabriel C . The dielectric properties of biological tissues: III. Parametric models for the dielectric spectrum of tissues. Phys Med Biol. 1996; 41(11):2271-93. DOI: 10.1088/0031-9155/41/11/003. View

13.

Iaccheri E, Ragni L, Cevoli C, Romani S, Dalla Rosa M, Rocculi P . Glass transition of green and roasted coffee investigated by calorimetric and dielectric techniques. Food Chem. 2019; 301:125187. DOI: 10.1016/j.foodchem.2019.125187. View

14.

Kruk D, Masiewicz E, Wojciechowski M, Florek-Wojciechowska M, Broche L, Lurie D . Slow dynamics of solid proteins - Nuclear magnetic resonance relaxometry versus dielectric spectroscopy. J Magn Reson. 2020; 314:106721. DOI: 10.1016/j.jmr.2020.106721. View

15.

Wang C, Che S, Susta L, Barbut S . Textural and physical properties of breast fillets with myopathies (wooden breast, white striping, spaghetti meat) in Canadian fast-growing broiler chickens. Poult Sci. 2022; 102(2):102309. PMC: 9720337. DOI: 10.1016/j.psj.2022.102309. View

16.

Petracci M, Laghi L, Rocculi P, Rimini S, Panarese V, Cremonini M . The use of sodium bicarbonate for marination of broiler breast meat. Poult Sci. 2012; 91(2):526-34. DOI: 10.3382/ps.2011-01753. View

17.

Sanden K, Bocker U, Ofstad R, Pedersen M, Host V, Afseth N . Characterization of Collagen Structure in Normal, Wooden Breast and Spaghetti Meat Chicken Fillets by FTIR Microspectroscopy and Histology. Foods. 2021; 10(3). PMC: 7998852. DOI: 10.3390/foods10030548. View

18.

Wolf M, Gulich R, Lunkenheimer P, Loidl A . Relaxation dynamics of a protein solution investigated by dielectric spectroscopy. Biochim Biophys Acta. 2012; 1824(5):723-30. DOI: 10.1016/j.bbapap.2012.02.008. View

19.

Schwan H, Foster K . Microwave dielectric properties of tissue. Some comments on the rotational mobility of tissue water. Biophys J. 1977; 17(2):193-7. PMC: 1473466. DOI: 10.1016/S0006-3495(77)85637-3. View

20.

Soglia F, Petracci M, Davoli R, Zappaterra M . A critical review of the mechanisms involved in the occurrence of growth-related abnormalities affecting broiler chicken breast muscles. Poult Sci. 2021; 100(6):101180. PMC: 8131729. DOI: 10.1016/j.psj.2021.101180. View