Natural Mutation in Stay-Green (OsSGR) Confers Enhanced Resistance to Rice Sheath Blight Through Elevating Cytokinin Content

Overview

Journal Plant Biotechnol J

Specialties Biology
Biotechnology

Date 2024 Dec 4

PMID 39630094

Authors

Wenya Xie

Xiang Xue

Yu Wang

Guiyun Zhang

Jianhua Zhao

Huimin Zhang

Guangda Wang

Lei Li

Yiqin Wang

Wenfeng Shan

Yafang Zhang

Zongxiang Chen

Xijun Chen

Zhiming Feng

Keming Hu

Mingfa Sun

Chengcai Chu

Shimin Zuo

Affiliations

Soon will be listed here.

Abstract

Sheath blight (ShB), caused by Rhizoctonia solani, is a highly destructive disease in many crops worldwide and no major resistance genes are available. Here, we identified a sbr1 (sheath blight resistance 1) rice mutant, which shows enhanced ShB resistance and maintains wildtype agronomic traits including yield, but carries an undesired stay-green phenotype. Through map-based cloning and transgenic validation, we found that an insertion disrupting the Stay-Green (OsSGR) gene is responsible for sbr1 phenotypes. Mechanistically, the sbr1/Ossgr mutants reduce the expression of most OsCKX genes, which function in cytokinin (CK) degradation, to accumulate CK leading to ShB resistance. Importantly, knockout of OsCKX7, predominantly expressed in the leaf sheath and highly induced by R. solani, significantly enhances ShB resistance without stay-green phenotype nor yield penalty, showing high application potential. Thus, our study reveals novel insights that OsSGR and cytokinin play key roles in rice-R. solani interaction and generates a valuable ShB-resistant germplasm.

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