Metabolic Engineering Strategies for Microbial Utilization of Methanol

Overview

Journal Eng Microbiol

Date 2024 Dec 4

PMID 39628934

Authors

Yamei Gan

Xin Meng

Cong Gao

Wei Song

Liming Liu

Xiulai Chen

Affiliations

Soon will be listed here.

Abstract

The increasing shortage of fossil resources and environmental pollution has renewed interest in the synthesis of value-added biochemicals from methanol. However, most of native or synthetic methylotrophs are unable to assimilate methanol at a sufficient rate to produce biochemicals. Thus, the performance of methylotrophs still needs to be optimized to meet the demands of industrial applications. In this review, we provide an in-depth discussion on the properties of natural and synthetic methylotrophs, and summarize the natural and synthetic methanol assimilation pathways. Further, we discuss metabolic engineering strategies for enabling microbial utilization of methanol for the bioproduction of value-added chemicals. Finally, we highlight the potential of microbial engineering for methanol assimilation and offer guidance for achieving a low-carbon footprint for the biosynthesis of chemicals.

Citing Articles

Enhancing D-lactic acid production by optimizing the expression of D-LDH gene in methylotrophic yeast Komagataella phaffii.

Inoue Y, Yamada R, Matsumoto T, Ogino H Biotechnol Biofuels Bioprod. 2024; 17(1):149.

PMID: 39710696 PMC: 11665205. DOI: 10.1186/s13068-024-02596-0.

References

Rohlhill J, Har J, Antoniewicz M, Papoutsakis E . Improving synthetic methylotrophy via dynamic formaldehyde regulation of pentose phosphate pathway genes and redox perturbation. Metab Eng. 2019; 57:247-255. DOI: 10.1016/j.ymben.2019.12.006. View

Krog A, Heggeset T, Muller J, Kupper C, Schneider O, Vorholt J . Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties. PLoS One. 2013; 8(3):e59188. PMC: 3602061. DOI: 10.1371/journal.pone.0059188. View

Adroer N, Casas C, de Mas C, Sola C . Mechanism of formaldehyde biodegradation by Pseudomonas putida. Appl Microbiol Biotechnol. 1990; 33(2):217-20. DOI: 10.1007/BF00176528. View

Chou A, Clomburg J, Qian S, Gonzalez R . 2-Hydroxyacyl-CoA lyase catalyzes acyloin condensation for one-carbon bioconversion. Nat Chem Biol. 2019; 15(9):900-906. DOI: 10.1038/s41589-019-0328-0. View

Witthoff S, Muhlroth A, Marienhagen J, Bott M . C1 metabolism in Corynebacterium glutamicum: an endogenous pathway for oxidation of methanol to carbon dioxide. Appl Environ Microbiol. 2013; 79(22):6974-83. PMC: 3811533. DOI: 10.1128/AEM.02705-13. View

Holscher T, Breuer U, Adrian L, Harms H, Maskow T . Production of the chiral compound (R)-3-hydroxybutyrate by a genetically engineered methylotrophic bacterium. Appl Environ Microbiol. 2010; 76(16):5585-91. PMC: 2918973. DOI: 10.1128/AEM.01065-10. View

Barrangou R, Doudna J . Applications of CRISPR technologies in research and beyond. Nat Biotechnol. 2016; 34(9):933-941. DOI: 10.1038/nbt.3659. View

Chistoserdova L, Lapidus A, Han C, Goodwin L, Saunders L, Brettin T . Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. J Bacteriol. 2007; 189(11):4020-7. PMC: 1913398. DOI: 10.1128/JB.00045-07. View

Nunez M, Pellicer M, Badia J, Aguilar J, Baldoma L . Biochemical characterization of the 2-ketoacid reductases encoded by ycdW and yiaE genes in Escherichia coli. Biochem J. 2001; 354(Pt 3):707-15. PMC: 1221703. DOI: 10.1042/0264-6021:3540707. View

10.

Peyraud R, Schneider K, Kiefer P, Massou S, Vorholt J, Portais J . Genome-scale reconstruction and system level investigation of the metabolic network of Methylobacterium extorquens AM1. BMC Syst Biol. 2011; 5:189. PMC: 3227643. DOI: 10.1186/1752-0509-5-189. View

11.

Dedysh S, Khmelenina V, Suzina N, Trotsenko Y, Semrau J, Liesack W . Methylocapsa acidiphila gen. nov., sp. nov., a novel methane-oxidizing and dinitrogen-fixing acidophilic bacterium from Sphagnum bog. Int J Syst Evol Microbiol. 2002; 52(Pt 1):251-261. DOI: 10.1099/00207713-52-1-251. View

12.

Yu H, Liao J . A modified serine cycle in Escherichia coli coverts methanol and CO to two-carbon compounds. Nat Commun. 2018; 9(1):3992. PMC: 6162302. DOI: 10.1038/s41467-018-06496-4. View

13.

Humphreys C, Minton N . Advances in metabolic engineering in the microbial production of fuels and chemicals from C1 gas. Curr Opin Biotechnol. 2018; 50:174-181. DOI: 10.1016/j.copbio.2017.12.023. View

14.

Wang Y, Fan L, Tuyishime P, Liu J, Zhang K, Gao N . Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum. Commun Biol. 2020; 3(1):217. PMC: 7205612. DOI: 10.1038/s42003-020-0954-9. View

15.

Meyer F, Keller P, Hartl J, Groninger O, Kiefer P, Vorholt J . Methanol-essential growth of Escherichia coli. Nat Commun. 2018; 9(1):1508. PMC: 5904121. DOI: 10.1038/s41467-018-03937-y. View

16.

Blazeck J, Alper H . Promoter engineering: recent advances in controlling transcription at the most fundamental level. Biotechnol J. 2012; 8(1):46-58. DOI: 10.1002/biot.201200120. View

17.

Kim S, Lindner S, Aslan S, Yishai O, Wenk S, Schann K . Growth of E. coli on formate and methanol via the reductive glycine pathway. Nat Chem Biol. 2020; 16(5):538-545. DOI: 10.1038/s41589-020-0473-5. View

18.

Liu Y, Li J, Du G, Chen J, Liu L . Metabolic engineering of Bacillus subtilis fueled by systems biology: Recent advances and future directions. Biotechnol Adv. 2016; 35(1):20-30. DOI: 10.1016/j.biotechadv.2016.11.003. View

19.

Antoniewicz M . Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production. Curr Opin Biotechnol. 2019; 59:165-174. DOI: 10.1016/j.copbio.2019.07.001. View

20.

Mo X, Zhang H, Wang T, Zhang C, Zhang C, Xing X . Establishment of CRISPR interference in Methylorubrum extorquens and application of rapidly mining a new phytoene desaturase involved in carotenoid biosynthesis. Appl Microbiol Biotechnol. 2020; 104(10):4515-4532. DOI: 10.1007/s00253-020-10543-w. View