Characterization of a Nitro-forming Oxygenase Involved in 3-(-2'-aminocyclopropyl)alanine Biosynthesis

Overview

Journal Eng Microbiol

Date 2024 Dec 4

PMID 39628616

Authors

Linlin Pang

Weijing Niu

Yuwei Duan

Liujie Huo

Aiying Li

Jiequn Wu

Youming Zhang

Xiaoying Bian

Guannan Zhong

Affiliations

Soon will be listed here.

Abstract

characterization experiments revealed the formations of 3-(-2'-aminocyclopropyl)alanine ((3-Acp)Ala) and 3-(-2'-nitrocyclopropyl)alanine ((3-Ncp)Ala) are originated via two homologous proteins, BelK and HrmI, which regioselectively catalyze the Nε-oxygenation of l-lysine. The two enzymes belong to the emerging heme-oxygenase-like diiron oxidase and oxygenase (HDO) superfamily and the catalytic center of BelK is validated by homology modeling and site-directed mutations. Based on the characterization, the biosynthetic pathways of (3-Acp)Ala and (3-Ncp)Ala are proposed.

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