Microbial Communities During the Composting Process of and Their Effects on Mushroom Agronomic and Nutritional Qualities

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Dec 2

PMID 39619689

Authors

Chunxia Wang

Dongxia Wang

Chao Li

Zhaopeng Ge

Liubin Hao

Gadah Albasher

Fan Feng

Yue Sun

Yanfen Lyu

Suyue Zheng

Affiliations

Soon will be listed here.

Abstract

Introduction: Tunnel composting technology for preparing cultivation media can achieve a higher biological efficiency (BE) and a lower contamination rate (CR). However, this technology lacks in-depth and systematic study.

Methods: In the present study, the changes in the microbiome and microbial metabolic functions were surveyed using metagenomic analysis. The physicochemical parameters, agronomic properties and nutritional qualities were also evaluated.

Results And Discussion: Results showed that the contents of cellulose, hemicellulose and lignin dropped to 10.18, 11.58, 27.53%, respectively at the end of composting. The tunnel composting technology led to significant increases in crude protein content (32.56%) and crude fiber content (13.68%). Variations of physicochemical characteristics led to different successions of microbial communities. Bacteria manifested significantly higher abundance than fungi. Firmicutes, Actinobacteriota, Chloroffexi and Deinococcota were the predominant bacterial phyla. Ascomycota and Basidiomycota were the dominant fungal phyla in the thermophilic phase. , , and were positively correlated with the yield of . In addition to TN, most of the physicochemical properties were significantly correlated with fungal communities in the thermophilic phase. The metabolisms of carbohydrate, amino acid and energy were the primary enrichment pathways. These findings deepen the understanding of microbial communities composition during the composting of substrates. Moreover, this study provides a basis for improving tunnel composting technology.

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