Involved in the Abscisic Acid Signaling Pathway to Regulate the Early Growth and Development of

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2024 Dec 2

PMID 39619177

Authors

Xiaoyang Xie

Lei Wei

Hongyuan Han

Bingnian Jing

Yuqing Liu

Yong Zhou

Ningjie Li

Xiao Li

Wei Wang

Affiliations

Soon will be listed here.

Abstract

Background: Living organisms possess the remarkable capacity to swiftly adapt to fluctuations in their environment. In the context of cell signal transduction, a significant challenge lies in ensuring the effective perception of external signals and the execution of appropriate responses. To investigate this phenomenon, a recent study utilized as a model plant and induced stress by administering abscisic acid (ABA), a plant hormone, to elucidate the involvement of leucine-rich repeat receptor-like kinase1 (LRR1) in ABA signaling pathways.

Methods: Homozygous T-DNA insertion alleles for and were isolated. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression of the gene. Subcellular localization and beta-glucuronidase (GUS) tissue labeling techniques were utilized to determine the expression pattern of the gene in cells and tissues. Yeast two-hybrid complementation, bimolecular fluorescence complementation assay, and GST pull-down assays were conducted to validate the interaction of LRR1 proteins.

Results: Phenotypic analyses revealed that and mutants are less sensitive to the inhibitory effects of ABA on germination and cotyledon greening that is seen in WT. Mutants and kinase 7 (KIN7) exhibited resistance to ABA and displayed normal growth patterns under control conditions. The double mutant showed reduced responsiveness to ABA. Conversely, overexpression lines and demonstrated heightened sensitivity to ABA, resulting in severe growth reduction. qRT-PCR assay indicated that exogenous application of ABA led to significant down-regulation of , , and transcription factors in material compared to wild-type WT material. An investigation was conducted to determine the expression pattern and transcriptional level of in Arabidopsis. The results revealed ubiquitous expression of across all developmental stages and tissue tested. Subcellular localization assays confirmed the presence of LRR1 on the plasma membrane of cells. Furthermore, BiFC assay, yeast two-hybrid complementation, and GST pull-down assays demonstrated an interaction between LRR1 and PYL6 . These findings provide substantial insights into the involvement of in the ABA signaling pathway while regulating seed germination and cotyledon greening during early development in Arabidopsis. This study significantly advances our understanding regarding the correlation between and ABA signaling pathways with potential applications for enhancing crop stress resistance.

References

Lozano-Elena F, Cano-Delgado A . Emerging roles of vascular brassinosteroid receptors of the BRI1-like family. Curr Opin Plant Biol. 2019; 51:105-113. DOI: 10.1016/j.pbi.2019.06.006. View

Utsugi S, Ashikawa I, Nakamura S, Shibasaka M . TaABI5, a wheat homolog of Arabidopsis thaliana ABA insensitive 5, controls seed germination. J Plant Res. 2020; 133(2):245-256. DOI: 10.1007/s10265-020-01166-3. View

Zhao C, Zayed O, Yu Z, Jiang W, Zhu P, Hsu C . Leucine-rich repeat extensin proteins regulate plant salt tolerance in . Proc Natl Acad Sci U S A. 2018; 115(51):13123-13128. PMC: 6305001. DOI: 10.1073/pnas.1816991115. View

Fujita Y, Fujita M, Shinozaki K, Yamaguchi-Shinozaki K . ABA-mediated transcriptional regulation in response to osmotic stress in plants. J Plant Res. 2011; 124(4):509-25. DOI: 10.1007/s10265-011-0412-3. View

Chen K, Li G, Bressan R, Song C, Zhu J, Zhao Y . Abscisic acid dynamics, signaling, and functions in plants. J Integr Plant Biol. 2019; 62(1):25-54. DOI: 10.1111/jipb.12899. View

Pan J, Hu Y, Wang H, Guo Q, Chen Y, Howe G . Molecular Mechanism Underlying the Synergetic Effect of Jasmonate on Abscisic Acid Signaling during Seed Germination in Arabidopsis. Plant Cell. 2020; 32(12):3846-3865. PMC: 7721325. DOI: 10.1105/tpc.19.00838. View

Hohmann U, Santiago J, Nicolet J, Olsson V, Spiga F, Hothorn L . Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors. Proc Natl Acad Sci U S A. 2018; 115(13):3488-3493. PMC: 5879659. DOI: 10.1073/pnas.1714972115. View

Man J, Gallagher J, Bartlett M . Structural evolution drives diversification of the large LRR-RLK gene family. New Phytol. 2020; 226(5):1492-1505. PMC: 7318236. DOI: 10.1111/nph.16455. View

Leung J, Merlot S, Giraudat J . The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell. 1997; 9(5):759-71. PMC: 156954. DOI: 10.1105/tpc.9.5.759. View

10.

Chen X, Wang T, Rehman A, Wang Y, Qi J, Li Z . Arabidopsis U-box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor-like protein kinases LRR1 and KIN7. J Integr Plant Biol. 2020; 63(3):494-509. DOI: 10.1111/jipb.13058. View

11.

Daszkowska-Golec A, Szarejko I . Open or close the gate - stomata action under the control of phytohormones in drought stress conditions. Front Plant Sci. 2013; 4:138. PMC: 3652521. DOI: 10.3389/fpls.2013.00138. View

12.

Park S, Fung P, Nishimura N, Jensen D, Fujii H, Zhao Y . Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science. 2009; 324(5930):1068-71. PMC: 2827199. DOI: 10.1126/science.1173041. View

13.

Baek W, Lim C, Lee S . A DEAD-box RNA helicase, RH8, is critical for regulation of ABA signalling and the drought stress response via inhibition of PP2CA activity. Plant Cell Environ. 2018; 41(7):1593-1604. DOI: 10.1111/pce.13200. View

14.

Wang Z, Ren Z, Cheng C, Wang T, Ji H, Zhao Y . Counteraction of ABA-Mediated Inhibition of Seed Germination and Seedling Establishment by ABA Signaling Terminator in Arabidopsis. Mol Plant. 2020; 13(9):1284-1297. DOI: 10.1016/j.molp.2020.06.011. View

15.

Fan M, Ma W, Liu C, Zhang C, Wu S, Chen M . Evolution and Expression Characteristics of Receptor-Like Cytoplasmic Protein Kinases in , and . Int J Mol Sci. 2018; 19(11). PMC: 6274858. DOI: 10.3390/ijms19113680. View

16.

Nagar P, Sharma N, Jain M, Sharma G, Prasad M, Mustafiz A . OsPSKR15, a phytosulfokine receptor from rice enhances abscisic acid response and drought stress tolerance. Physiol Plant. 2021; 174(1):e13569. DOI: 10.1111/ppl.13569. View

17.

Ghidoli M, Ponzoni E, Araniti F, Miglio D, Pilu R . Genetic Improvement of (L.) Crantz: Opportunities and Challenges. Plants (Basel). 2023; 12(3). PMC: 9920110. DOI: 10.3390/plants12030570. View

18.

Liu Y, Jiang M, Li R, Huang J, Shu Q . OsKEAP1 Interacts with OsABI5 and Its Downregulation Increases the Transcription of and the ABA Response Genes in Germinating Rice Seeds. Plants (Basel). 2021; 10(3). PMC: 8001349. DOI: 10.3390/plants10030527. View

19.

Fiesselmann B, Luichtl M, Yang X, Matthes M, Peis O, Torres-Ruiz R . Ectopic shoot meristem generation in monocotyledonous rpk1 mutants is linked to SAM loss and altered seedling morphology. BMC Plant Biol. 2015; 15:171. PMC: 4492102. DOI: 10.1186/s12870-015-0556-8. View

20.

Guo H, Nolan T, Song G, Liu S, Xie Z, Chen J . FERONIA Receptor Kinase Contributes to Plant Immunity by Suppressing Jasmonic Acid Signaling in Arabidopsis thaliana. Curr Biol. 2018; 28(20):3316-3324.e6. DOI: 10.1016/j.cub.2018.07.078. View