Untargeted Serum Metabonomic Reveals Alleviated Ovalbumin-induced Asthma by Baijin Pingchuan Through Primary Bile Acid Biosynthesis

Overview

Journal J Tradit Chin Med

Date 2024 Dec 1

PMID 39617704

Authors

Ding Lizhong

Zhang Qiang

Sun Yingying

Kong Yibu

Song Yongfu

Wang Yongji

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the effect of baijinpingchuan (, BJPC) on the asthma rat model and identify differential metabolites and disturbed metabolic pathways.

Methods: The rats were categorized into six groups: control, dexamethasone (DEX), ovalbumin (OVA), and low-, median-, and high-dose BJPC. The rats, except for the control group, were initially treated with OVA to develop the asthma model, which was then activated using DEX, OVA, and low-, median-, and high-dose BJPC. Enzyme-linked immunosorbent assay kit was used to detect the expression of interleukin (IL)-33, IL-25, thymic stromal lymphopoietin (TSLP), and transforming growth factor-beta 1 (TGF-β1). Hematoxylin and eosin staining were performed to observe the pathological condition of the lung. Untargeted serum metabonomic analysis was conducted to identify differential metabolites and disturbed metabolic pathways.

Results: High-dose BJPC significantly inhibited the expression of IL-33, IL-25, TSLP, and TGF-β1 ( 0.0001). Further, high-dose BJPC improved inflammatory cell infiltration, which plays a similar role in asthma as DEX. OVA-induced and BJPC-treated rats were identified through 17 differential metabolites, especially cholic acid. Furthermore, primary bile acid biosynthesis was a significantly differential pathway in the mechanism of BJPC for treating asthma.

Conclusions: BJPC plays an anti-inflammation role in asthma, which might be a promising therapy through mediating primary bile acid biosynthesis.

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