In Vitro Activity of Nanoliposomal Amphotericin B Against Terbinafine-resistant Trichophyton Indotineae Isolates

Background: The emergence of resistance in dermatophytes underscores the necessity for developing novel and alternative treatment options.

Methods: The present study aimed to evaluate the in vitro activity of nanoliposomal amphotericin B against a large panel of terbinafine-resistant Trichophyton indotineae isolates. In vitro susceptibility testing of nanoliposomal amphotericin B and comparators against 50 clinical isolates of terbinafine-resistant T. indotineae strains was performed by broth microdilution according to the Clinical and Laboratory Standards Institute (CLSI) M38-A3 document.

Results: The highest MIC and widest MIC range were found for terbinafine (4 and ≥ 4 µg/ml) followed by itraconazole (0.5 and 0.016-16 µg/ml). In contrast, the nanoliposomal amphotericin B and conventional amphotericin B exhibited low MIC (0.5 µg/ml) and MIC range (0.25-0.5 µg/ml).

Conclusions: The study findings revealed that nanoliposomal amphotericin B might be a promising candidate against terbinafine-resistant strains of T. indotineae. However, further studies are required to pave the way for developing novel antifungal compounds to combat fungal resistance.