β-hydroxybutyrate Suppresses Pathological Changes of Blood-induced Arthropathy in Rats

Overview

Journal Sci Rep

Specialty Science

Date 2024 Nov 29

PMID 39614095

Authors

Ryohei Kawasaki

Asuka Sakata

Kohei Tatsumi

Seiji Mitani

Maiko Takeda

Shogo Kasuda

Naoki Matsumoto

Suguru Harada

Tetsuhiro Soeda

Yukiko Nishida

Yasushi Yoshimura

Midori Shima

Affiliations

Soon will be listed here.

Abstract

Arthropathy is a common complication in haemophilia and decreases quality of life. It has been known that concentrations of β-hydroxybutyrate (BHB) in blood are increased by a ketogenic diet, and elevated levels of circulating BHB restricts the progression of inflammation-mediated joint pathological changes. We hypothesized that elevation of blood BHB concentrations could be effective for reducing the progression of bleeding-induced arthropathy by moderating the inflammatory responses of macrophages. In this study, we investigated whether BHB alleviates the arthropathy caused by repeated intra-articular blood injection in rats. To increase blood BHB levels, rats were fed with ketogenic diet. Repeated intra-articular blood injection induced significant joint swelling, whereas ketogenic diet intake significantly increased blood BHB concentrations and ameliorated the joint swelling. The periarticular tissue-fibrosis observed in the control diet intake group appeared to be significantly alleviated in the ketogenic diet intake group. In addition, the IL-1β, which is involved in the progression of arthropathy, levels in the supernatants of blood-exposed macrophages derived from THP-1 cell line were significantly suppressed by BHB supplementation. In summary, BHB moderated the pathological joint changes caused by intra-articular blood exposure.

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