Detection of Drug Resistance in from Calves with Diarrhea in the Tongliao Region: an Analysis of Multidrug-resistant Strains

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2024 Nov 28

PMID 39606646

Authors

Zi Wang

Miao Sun

Shuang Guo

Yongqiang Wang

Linghao Meng

Jinchuan Shi

Chao Geng

Dongxu Han

Xiaomeng Fu

Jiangdong Xue

Hongxia Ma

Kai Liu

Affiliations

Soon will be listed here.

Abstract

Introduction: is a major pathogen responsible for calf diarrhea, which has been exacerbated by the irrational and unscientific use of antimicrobial drugs, leading to significant drug resistance.

Methods: This study focused on the isolation and identification of from calf diarrhea samples in the Tongliao area of China. Isolation was conducted using selective media, Gram staining, and 16S rRNA sequencing. The minimum inhibitory concentration (MIC) of was determined through the microbroth dilution method. Additionally, the presence of antibiotic-resistant genes was detected, and multidrug-resistant strains were selected for whole-genome sequencing (WGS).

Results: The results revealed that all 40 isolated strains of exhibited resistance to sulfadiazine sodium, enrofloxacin, and ciprofloxacin, with 90% of the strains being susceptible to polymyxin B. Notably, strains 11, 23, and 24 demonstrated severe resistance. The detection rates of the antibiotic resistance genes , , , , and were 100%, indicating a high prevalence of these genes. Moreover, the majority of strains carried antibiotic resistance genes consistent with their resistance phenotypes. WGS of strains 11, 23, and 24 revealed genome sizes of 4,897,185 bp, 4,920,234 bp, and 4,912,320 bp, respectively. These strains carried two, one, and two plasmids, respectively. The prediction of antibiotic resistance genes showed a substantial number of these genes within the genomes, with strain 24 harboring the highest number, totaling 77 subspecies containing 88 antibiotic resistance genes.

Discussion: In conclusion, all 40 isolated strains of E. coli from calf diarrhea in this study were multidrug-resistant, exhibiting a broad distribution of antibiotic resistance genes and mobile components. This poses a significant risk of horizontal gene transfer, highlighting the critical situation of antibiotic resistance in this region.

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