Genome-wide Characterization and Comparative Expression Profiling of Genes in Yellow Catfish () After Infection with Exogenous

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2024 Nov 28

PMID 39606227

Authors

Shengtao Guo

Mengsha Zeng

Chenhao Zhang

Yuxin Fan

Miling Ran

Zhaobin Song

Affiliations

Soon will be listed here.

Abstract

Introduction: Dual-specificity phosphatases (DUSPs) are crucial regulators in many mammals, managing dephosphorylation and inactivation of mitogen-activated protein kinases (MAPKs) and playing essential roles in immune responses. However, their presence and functions in teleosts, like the yellow catfish (), remain unexplored.

Methods: In this study, eight genes ( and ) were identified in yellow catfish. We characterized their molecular features, conserved protein sequences, and chromosomal localization through genome-wide analyses, and we examined their expression patterns in immune responses.

Results: Our findings reveal two conserved motifs, Leu-Phe-Leu-Gly and Ala-Tyr-Leu-Met, within the DSPc domain of DUSP proteins. The genes were mapped across seven chromosomes without evidence of duplication. Comparative analysis showed high conservation of Dusp genes across vertebrates, with evolutionary analysis suggesting as a potential intermediate form. transcripts were significantly upregulated in the kidney post- infection.

Discussion: These results suggest the involvement of genes in the immune response of yellow catfish to bacterial pathogens, providing insights into their evolutionary significance and potential applications in aquaculture and molecular breeding.

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