Mechanism of a Novel JNF2 in Suppressing F. Sp. and Enhancing Cucumber Growth

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Nov 28

PMID 39606119

Authors

Fan Yang

Xin Wang

Huayan Jiang

Qiuju Yao

Shen Liang

Weiwei Chen

Gongyao Shi

Baoming Tian

Abeer Hegazy

Shengli Ding

Affiliations

Soon will be listed here.

Abstract

Cucumber wilt caused by f. sp. (), is a prevalent soil-borne disease. In this study, JNF2, isolated from the high incidence area of cucumber wilt in Luoyang, demonstrated significant inhibitory effects on and promoted cucumber seedling growth. The biocontrol mechanism of strain JNF2 were elucidated through morphological observation, physiological and biochemical experiments, and whole genome sequence analysis. Pot experiments revealed an 81.33 ± 0.21% control efficacy against wilt, surpassing the 64.10 ± 0.06% efficacy of hymexazol. Seedlings inoculated with JNF2 exhibited enhanced stem thickness and leaf area compared to control and hymexazol-treated plants. Physiological tests confirmed JNF2's production of indole-3-acetic acid (IAA), siderophores, and hydrolytic enzymes, such as β-1,3-glucanase, amylase, and protease, which inhibited growth and promoted plant development. Genome analysis identified genes encoding antimicrobial peptides and hydrolases, as well as a novel glycocin synthetic gene cluster. These findings underscore JNF2's potential as a biocontrol agent for sustainable cucumber cultivation.

Citing Articles

Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber Wilt.

Yang F, Wang X, Jiang H, Chang X, Chen W, Shi G Microorganisms. 2025; 13(1).

PMID: 39858901 PMC: 11767639. DOI: 10.3390/microorganisms13010133.

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