Characterization of the T-cell Receptor Repertoire Associated with Lymph Node Metastasis in Colorectal Cancer

Overview

Journal Front Oncol

Specialty Oncology

Date 2024 Nov 27

PMID 39600636

Authors

Yanan Zhen

Hong Wang

Runze Jiang

Fang Wang

Cunbao Chen

Zhongfa Xu

Ruixue Xiao

Affiliations

Soon will be listed here.

Abstract

Purpose: Colorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide, with lymph node (LN) metastasis playing a pivotal role in disease progression. This study aimed to explore the T-cell receptor (TCR) repertoire among CRC patients, distinguishing those with LN metastasis from those without, in order to uncover potential biomarkers for predicting metastasis.

Methods: We analyzed the TCR repertoire in CRC patients with and without LN metastasis. A classification model utilizing random forest analysis was developed to assess the predictive potential of the TCR repertoire.

Results: The findings demonstrated a significant increase in the number of V-J combinations and immune CDR3 sequences in patients with LN metastasis compared to the control group. The classification model achieved high accuracy in differentiating patients with LN metastasis, with AUC values ranging from 0.514 to 0.794. Specific V-J combinations and CDR3 sequences were identified as significant predictors of the model's predictive accuracy.

Conclusion: These results suggest that the TCR repertoire is altered in CRC patients exhibiting LN metastasis, potentially influencing disease progression. This study highlights the importance of TCR repertoire analysis as a non-invasive biomarker for predicting LN metastasis in CRC patients.

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