Immune Response Modulation by HPV16 Oncoproteins in Lung Cancer: Insights from Clinical and In Vitro Investigations

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2024 Nov 27

PMID 39599846

Authors

Bianca de Franca Sao Marcos

Daffany Luana Dos Santos

Georon Ferreira de Sousa

Leonardo Carvalho de Oliveira Cruz

Barbara Rafaela da Silva Barros

Matheus Gardini Amancio Marques de Sena

Vanessa Emanuelle Pereira Santos

Talita Helena de Araujo Oliveira

Cristiane Moutinho Lagos de Melo

Antonio Carlos de Freitas

Affiliations

Soon will be listed here.

Abstract

Lung cancer has the highest mortality rates worldwide, and Human Papillomavirus (HPV) has been associated with its carcinogenesis. In this study, HPV16 genes' expressions were investigated in patient samples, along with the immunological response promoted by lymphocytes and monocytes in A549 cells transfected with HPV oncogenes and co-cultured with PBMC. An increase in the expression of E5 was observed in the patients' samples. In the in vitro analysis, a decrease in the number of monocytes and cytotoxic cells was observed when co-stimulated by E6 and E7, and it promoted an increase in the Th2 profile. In contrast, the high proliferation of cytotoxic cells in A549 cells transfected with E5, associated with the high expression of costimulatory molecules in monocytes, suggests a low capacity of E5 to inhibit the presentation of antigens by antigen-presenting cells (APC) and a possible use of E5 in future therapeutic strategies against lung cancers associated with HPV.

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