RNA-Seq Reveals Transcriptome Changes Following Zika Virus Infection in Fetal Brains in Knockdown Mice

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2024 Nov 27

PMID 39599827

Authors

Ting Xie

Qiqi Chen

Nina Li

Shengze Zhang

Lin Zhu

Shaohui Bai

Haolu Zha

Weijian Tian

Chuming Luo

Nan Wu

Xuan Zou

Shisong Fang

Yuelong Shu

Jianhui Yuan

Ying Jiang

Huanle Luo

Affiliations

Soon will be listed here.

Abstract

The FADD-like interleukin-1β converting enzyme (FLICE)-inhibitory protein (c-FLIP) plays a crucial role in various biological processes, including apoptosis and inflammation. However, the complete transcriptional profile altered by the c-FLIP is not fully understood. Furthermore, the impact of the c-FLIP deficiency on the transcriptome during a Zika virus (ZIKV) infection, which induces apoptosis and inflammation in the central nervous system (CNS), has not yet been elucidated. In this study, we compared transcriptome profiles between wild-type (WT) and the heterozygous knockout mice () fetal heads at embryonic day 13.5 from control and PBS-infected WT dams mated with sires. In the non-infected group, we observed differentially expressed genes (DEGs) mainly involved in embryonic development and neuron development. However, the ZIKV infection significantly altered the transcriptional profile between WT and the fetal heads. DEGs in pattern recognition receptor (PRR)-related signaling pathways, such as the RIG-I-like receptor signaling pathway and Toll-like receptor signaling pathway, were enriched. Moreover, the DEGs were also enriched in T cells, indicating that the c-FLIP participates in both innate and adaptive immune responses upon viral infection. Furthermore, our observations indicate that DEGs are associated with sensory organ development and eye development, suggesting a potential role for the c-FLIP in ZIKV-induced organ development defects. Overall, we have provided a comprehensive transcriptional profile for the c-FLIP and its modulation during a ZIKV infection.

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