Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2024 Nov 27

PMID 39596812

Authors

Rakhim Kanat

Malika Shamekova

Zagipa Sapakhova

Maxat Toishimanov

Dias Daurov

Nurgul Raissova

Zhanar Abilda

Ainash Daurova

Kabyl Zhambakin

Affiliations

Soon will be listed here.

Abstract

Drought has increasingly affected the yield of L. (potato) every year over the last decade, posing serious economic problems for the global agricultural industry. Therefore, it is important to research drought tolerance in plants and obtain more robust varieties of crops. The aim of the present work was to study the expression of drought-upregulated genes in drought-tolerant and drought-sensitive varieties of potato. Bioreactors were used to identify whether each variety was drought-tolerant or drought-sensitive; then, expression analysis was performed according to the morphological characteristics of the plantlets in two different media: Murashige and Skoog (MS) medium and MS medium with 20% PEG-6000 to simulate osmotic stress. Based on the quantitative parameters of six initial varieties, two varieties were selected (Gala and Aksor) for further gene expression analysis. The expression of genes commonly upregulated in drought (, , , , and ) was higher in the drought-tolerant variety than in the sensitive one. Therefore, the expression of these genes can be used to determine the drought tolerance of a potato variety in vitro in the early plant development stage. Moreover, comparative analysis showed that some of the targeted genes used to identify drought tolerance in this study are conserved across different plant species.

References

Li Q, Qin Y, Hu X, Li G, Ding H, Xiong X . Transcriptome analysis uncovers the gene expression profile of salt-stressed potato (Solanum tuberosum L.). Sci Rep. 2020; 10(1):5411. PMC: 7096413. DOI: 10.1038/s41598-020-62057-0. View

Brown J, Pirrung M, McCue L . FQC Dashboard: integrates FastQC results into a web-based, interactive, and extensible FASTQ quality control tool. Bioinformatics. 2017; 33(19):3137-3139. PMC: 5870778. DOI: 10.1093/bioinformatics/btx373. View

Schumacher C, Krannich C, Maletzki L, Kohl K, Kopka J, Sprenger H . Unravelling Differences in Candidate Genes for Drought Tolerance in Potato ( L.) by Use of New Functional Microsatellite Markers. Genes (Basel). 2021; 12(4). PMC: 8067248. DOI: 10.3390/genes12040494. View

Pertea M, Pertea G, Antonescu C, Chang T, Mendell J, Salzberg S . StringTie enables improved reconstruction of a transcriptome from RNA-seq reads. Nat Biotechnol. 2015; 33(3):290-5. PMC: 4643835. DOI: 10.1038/nbt.3122. View

Zhang Z, Li F, Li D, Zhang H, Huang R . Expression of ethylene response factor JERF1 in rice improves tolerance to drought. Planta. 2010; 232(3):765-74. DOI: 10.1007/s00425-010-1208-8. View

Davies W, Wilkinson S, Loveys B . Stomatal control by chemical signalling and the exploitation of this mechanism to increase water use efficiency in agriculture. New Phytol. 2021; 153(3):449-460. DOI: 10.1046/j.0028-646X.2001.00345.x. View

Chen Y, Li C, Yi J, Yang Y, Lei C, Gong M . Transcriptome Response to Drought, Rehydration and Re-Dehydration in Potato. Int J Mol Sci. 2019; 21(1). PMC: 6981527. DOI: 10.3390/ijms21010159. View

Wang J, Yang F, Chen X, Liang R, Zhang L, Geng D . Induced expression of DREB transcriptional factor and study on its physiological effects of drought tolerance in transgenic wheat. Yi Chuan Xue Bao. 2006; 33(5):468-76. DOI: 10.1016/S0379-4172(06)60074-7. View

Han X, Tang S, An Y, Zheng D, Xia X, Yin W . Overexpression of the poplar NF-YB7 transcription factor confers drought tolerance and improves water-use efficiency in Arabidopsis. J Exp Bot. 2013; 64(14):4589-601. PMC: 3808328. DOI: 10.1093/jxb/ert262. View

10.

Saidi M, Mahjoubi H, Yacoubi I . Transcriptome meta-analysis of abiotic stresses-responsive genes and identification of candidate transcription factors for broad stress tolerance in wheat. Protoplasma. 2022; 260(3):707-721. DOI: 10.1007/s00709-022-01807-5. View

11.

Tang X, Zhang N, Si H, Calderon-Urrea A . Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress. Plant Methods. 2017; 13:85. PMC: 5644265. DOI: 10.1186/s13007-017-0238-7. View

12.

Chen J, Meng X, Zhang Y, Xia M, Wang X . Over-expression of OsDREB genes lead to enhanced drought tolerance in rice. Biotechnol Lett. 2008; 30(12):2191-8. DOI: 10.1007/s10529-008-9811-5. View

13.

Zhang Y, Park C, Bennett C, Thornton M, Kim D . Rapid and accurate alignment of nucleotide conversion sequencing reads with HISAT-3N. Genome Res. 2021; 31(7):1290-1295. PMC: 8256862. DOI: 10.1101/gr.275193.120. View

14.

Yan C, Song S, Wang W, Wang C, Li H, Wang F . Screening diverse soybean genotypes for drought tolerance by membership function value based on multiple traits and drought-tolerant coefficient of yield. BMC Plant Biol. 2020; 20(1):321. PMC: 7346468. DOI: 10.1186/s12870-020-02519-9. View

15.

Gong L, Zhang H, Gan X, Zhang L, Chen Y, Nie F . Transcriptome Profiling of the Potato (Solanum tuberosum L.) Plant under Drought Stress and Water-Stimulus Conditions. PLoS One. 2015; 10(5):e0128041. PMC: 4444143. DOI: 10.1371/journal.pone.0128041. View

16.

Liu M, Yu H, Zhao G, Huang Q, Lu Y, Ouyang B . Profiling of drought-responsive microRNA and mRNA in tomato using high-throughput sequencing. BMC Genomics. 2017; 18(1):481. PMC: 5485680. DOI: 10.1186/s12864-017-3869-1. View

17.

Jian H, Sun H, Liu R, Zhang W, Shang L, Wang J . Construction of drought stress regulation networks in potato based on SMRT and RNA sequencing data. BMC Plant Biol. 2022; 22(1):381. PMC: 9341072. DOI: 10.1186/s12870-022-03758-8. View

18.

Munoz-Mayor A, Pineda B, Garcia-Abellan J, Anton T, Garcia-Sogo B, Sanchez-Bel P . Overexpression of dehydrin tas14 gene improves the osmotic stress imposed by drought and salinity in tomato. J Plant Physiol. 2012; 169(5):459-68. DOI: 10.1016/j.jplph.2011.11.018. View

19.

Jung C, Nguyen N, Cheong J . Transcriptional Regulation of Protein Phosphatase 2C Genes to Modulate Abscisic Acid Signaling. Int J Mol Sci. 2020; 21(24). PMC: 7765119. DOI: 10.3390/ijms21249517. View

20.

Dobranszki J, Magyar-Tabori K, Takacs-Hudak A . Growth and developmental responses of potato to osmotic stress under in vitro conditions. Acta Biol Hung. 2004; 54(3-4):365-72. DOI: 10.1556/ABiol.54.2003.3-4.14. View