Novel Allelic Mutations in Gene That Affect the Height of Sorghum Plants

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Nov 27

PMID 39596067

Authors

Ping Wang

Bingbing Liang

Zhengjun Li

Chunyu Wang

Lixia Zhang

Xiaochun Lu

Affiliations

Soon will be listed here.

Abstract

Breeding for dwarfing traits in sorghum is crucial. However, only three genes (-) that control plant height have been mapped. In this study, 634 sorghum cultivars were collected to investigate plant height and genotypes. Four were genotyped (wild type) but with different plant heights, and they were selected to construct two populations and map new dwarf genes. Bulked segregant analysis with whole-genome resequencing of the two populations identified the candidate gene in one same genomic region-on chromosome 7. Then, it was narrowed down to a region containing nine genes. Amino acid and DNA sequence analysis of the parent and offspring plants revealed that two novel allelic mutations in the gene play a role in reducing the plant height-8R262 or 8R417, including 1 bp substitution and 2 bp deletions. Furthermore, we sequenced 19 cultivars that primarily exhibited a "one-dwarf" hybrid or wild-type and presumed another allelic mutation via the amino acid alignment of 8R019, 8R100, and 8R402, which was another one-base substitution. These results indicate that multiple types of allelic mutations in the gene should be considered when identified or applied.

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