Bioactive Hybrids Containing Artificial Cell Membranes and Phyto-Gold-Silver Chloride Bio-Nanoparticles

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Nov 27

PMID 39596000

Authors

Marcela-Elisabeta Barbinta-Patrascu

Cornelia Nichita

Monica Enculescu

Valentin-Adrian Maraloiu

Mihaela Bacalum

Camelia Ungureanu

Catalin Constantin Negrila

Irina Zgura

Affiliations

Soon will be listed here.

Abstract

This research targets the need for eco-friendly strategies in the synthesis of bioactive materials, addressing the importance of valorization of vegetal waste. This study focuses on developing biohybrids containing biomimetic lipid vesicles and phytosynthesized gold-silver chloride nanoparticles (AuAgCl NPs) derived from L. extract. By leveraging the natural antioxidant and antimicrobial properties of the plant, the research proposes a sustainable approach to creating materials with potential biomedical applications. The biomimetic membranes were loaded with chlorophyll , a natural spectral marker. Three types of bioactive materials (biohybrids) were developed by varying the lipid vesicle/AuAgCl NP ratio. Optical (UV-Vis, fluorescence emission, FTIR), structural (XRD), elemental (EDX, XPS), and morphological (TEM) studies were performed to characterize the bio-developed materials. The hydrophobic/hydrophilic characteristics of the samples were investigated by measuring the water contact angle, and their size was estimated by DLS and TEM. Zeta potential measurements were used to evaluate the physical stability of phyto-developed particles. Antioxidant properties of phyto-particles were investigated through the chemiluminescence technique. The obtained biomaterials exhibited high antioxidant activity and antiproliferative activity against HT-29 and B-16 cancer cells. Therapeutic index values were calculated for each biohybrid. Additionally, the bio-prepared hybrids revealed biocidal action against and . The phyto-developed biomaterials are promising in biomedical applications, particularly as adjuvants in cancer therapy.

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