A Novel Single Base Mutation in Leads to the Formation of Leaf Lesions in Rice

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Nov 27

PMID 39595944

Authors

Panpan Li

Huihui Shang

Xia Xu

Junyi Gong

Jian-Li Wu

Xiaobo Zhang

Affiliations

Soon will be listed here.

Abstract

Rice spotted-leaf mutants serve as valuable resources for studying plant programmed cell death (PCD) and disease resistance mechanisms, making them crucial for research on disease resistance in rice. Map-based cloning was used to identify and clone the spotted-leaf gene . Then, functional complementation and CRISPR/Cas9 techniques were also employed to further validate the function of this gene. By applying leaf clippings for bacterial blight (BB) inoculation, the BB resistance of different rice lines was assessed. The results in this study were as follows: The behaved as a recessive nuclear gene and was narrowed down to a 111 kb region on chromosome 8. All T transgenic rice plants in the complementation experiments exhibited a wild-type phenotype, without any lesion spots on the rice leaves. This suggests that the encoding O-methyltransferase is the candidate gene for the mutant . The is widely expressed and the OsSPL42-GFP protein is mainly localized in the cytoplasm. overexpression lines are more susceptible to BBs, which indicates that may act as a negative regulator of rice resistance to BB. In summary, we speculate that plays an important role in the regulation of pathogen response, providing new insights into plant defense mechanisms.

References

Jung K, Cao P, Seo Y, Dardick C, Ronald P . The Rice Kinase Phylogenomics Database: a guide for systematic analysis of the rice kinase super-family. Trends Plant Sci. 2010; 15(11):595-9. DOI: 10.1016/j.tplants.2010.08.004. View

Liang S, Xu S, Qu D, Yang L, Wang J, Liu H . Identification and Functional Analysis of the Caffeic Acid O-Methyltransferase (COMT) Gene Family in Rice ( L.). Int J Mol Sci. 2022; 23(15). PMC: 9369235. DOI: 10.3390/ijms23158491. View

Chen Z, Chen T, Sathe A, He Y, Zhang X, Wu J . Identification of a Novel Semi-Dominant Spotted-Leaf Mutant with Enhanced Resistance to pv. in Rice. Int J Mol Sci. 2018; 19(12). PMC: 6321207. DOI: 10.3390/ijms19123766. View

Yang Y, Xu J, Huang L, Leng Y, Dai L, Rao Y . PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice. J Exp Bot. 2015; 67(5):1297-310. PMC: 4762379. DOI: 10.1093/jxb/erv529. View

Hiei Y, Ohta S, Komari T, Kumashiro T . Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J. 1994; 6(2):271-82. DOI: 10.1046/j.1365-313x.1994.6020271.x. View

Cao J, Jiang F, Sodmergen , Cui K . Time-course of programmed cell death during leaf senescence in Eucommia ulmoides. J Plant Res. 2003; 116(1):7-12. DOI: 10.1007/s10265-002-0063-5. View

Bouchez O, Huard C, Lorrain S, Roby D, Balague C . Ethylene is one of the key elements for cell death and defense response control in the Arabidopsis lesion mimic mutant vad1. Plant Physiol. 2007; 145(2):465-77. PMC: 2048732. DOI: 10.1104/pp.107.106302. View

Zhao D, Luan Y, Shi W, Zhang X, Meng J, Tao J . A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging. Plant Sci. 2021; 303:110765. DOI: 10.1016/j.plantsci.2020.110765. View

Arnon D . COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949; 24(1):1-15. PMC: 437905. DOI: 10.1104/pp.24.1.1. View

10.

Zhang X, Chen B, Wang L, Ali S, Guo Y, Liu J . Genome-Wide Identification and Characterization of Gene Family in Soybean. Plants (Basel). 2021; 10(12). PMC: 8703356. DOI: 10.3390/plants10122816. View

11.

Kurata N, Miyoshi K, Nonomura K, Yamazaki Y, Ito Y . Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiol. 2005; 46(1):48-62. DOI: 10.1093/pcp/pci506. View

12.

Ma J, Wang Y, Ma X, Meng L, Jing R, Wang F . Disruption of gene SPL35, encoding a novel CUE domain-containing protein, leads to cell death and enhanced disease response in rice. Plant Biotechnol J. 2019; 17(8):1679-1693. PMC: 6662554. DOI: 10.1111/pbi.13093. View

13.

Huang Q, Shi Y, Yang Y, Feng B, Wei Y, Chen J . Characterization and genetic analysis of a light- and temperature-sensitive spotted-leaf mutant in rice. J Integr Plant Biol. 2011; 53(8):671-81. DOI: 10.1111/j.1744-7909.2011.01056.x. View

14.

Zurbriggen M, Carrillo N, Hajirezaei M . ROS signaling in the hypersensitive response: when, where and what for?. Plant Signal Behav. 2010; 5(4):393-6. PMC: 2958590. DOI: 10.4161/psb.5.4.10793. View

15.

Shang H, Li P, Zhang X, Xu X, Gong J, Yang S . The Gain-of-Function Mutation, Positively Regulates Plant Immunity in Rice. Int J Mol Sci. 2022; 23(22). PMC: 9697700. DOI: 10.3390/ijms232214168. View

16.

Hirano K, Aya K, Kondo M, Okuno A, Morinaka Y, Matsuoka M . OsCAD2 is the major CAD gene responsible for monolignol biosynthesis in rice culm. Plant Cell Rep. 2011; 31(1):91-101. DOI: 10.1007/s00299-011-1142-7. View

17.

Keisa A, Kanberga-Silina K, Nakurte I, Kunga L, Rostoks N . Differential disease resistance response in the barley necrotic mutant nec1. BMC Plant Biol. 2011; 11:66. PMC: 3089796. DOI: 10.1186/1471-2229-11-66. View

18.

Huangfu L, Chen R, Lu Y, Zhang E, Miao J, Zuo Z . OsCOMT, encoding a caffeic acid O-methyltransferase in melatonin biosynthesis, increases rice grain yield through dual regulation of leaf senescence and vascular development. Plant Biotechnol J. 2022; 20(6):1122-1139. PMC: 9129082. DOI: 10.1111/pbi.13794. View

19.

Heath M . Nonhost resistance and nonspecific plant defenses. Curr Opin Plant Biol. 2000; 3(4):315-9. DOI: 10.1016/s1369-5266(00)00087-x. View

20.

Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R . A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. Mol Plant. 2015; 8(8):1274-84. DOI: 10.1016/j.molp.2015.04.007. View