Effects of Nitrite Stress on the Antioxidant, Immunity, Energy Metabolism, and Microbial Community Status in the Intestine of

Overview

Journal Antioxidants (Basel)

Date 2024 Nov 27

PMID 39594461

Authors

Yafei Duan

Guowei Zhong

Yuxiu Nan

Yukai Yang

Meng Xiao

Hua Li

Affiliations

Soon will be listed here.

Abstract

Nitrite is the main environmental pollutant that endangers shrimp culture. Intestinal health is essential for the disease resistance of shrimp. In this study, shrimps were separately exposed to 1 and 5 mg/L of nitrite stress for 48 h, and then the variations in intestinal health were investigated from the aspects of histology, antioxidant, immunity, energy metabolism, and microbial community status. The results showed that nitrite stress damaged intestinal mucosa, and 5 mg/L of nitrite induced more obvious physiological changes than 1 mg/L. Specifically, the relative expression levels of antioxidant (, , , and ), ER stress ( and ), immunity (, , , and ), inflammation ( and ), and apoptosis ( and ) genes were increased. Additionally, intestinal energy metabolism was activated by inducing glucose metabolism (, , , and ), lipid metabolism ( and ), tricarboxylic acid cycle (, , , , and ), and electron transfer chain (, , , and ) gene transcription. Further, the homeostasis of intestinal microbiota composition was also disturbed, especially the abundance of some beneficial genera (, , , and ). These results reveal that nitrite stress could damage the intestinal health of by destroying mucosal integrity, inducing oxidation and ER stress, interfering with physiological homeostasis and energy metabolism, and disrupting the microbial community.

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