Immunomodulatory Role of Trichinella Spiralis-derived Antigen on Imiquimod-induced Psoriasis in Mice Model

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2024 Nov 26

PMID 39592463

Authors

Nahla El Skhawy

Maha M Eissa

Maram Allam

Eman M Eleryan

Affiliations

Soon will be listed here.

Abstract

The immunomodulatory activity of parasites has been extensively investigated in multiple immune-related diseases. However, dermatological diseases have been off the list for a long time despite their vast incidence and the deleterious consequences of some of them. This study explored the immunomodulatory role of autoclaved Trichinella spiralis (T. spiralis) larvae antigen (ATSLA) as a psoriasis immunotherapeutic candidate in a mice model. Psoriasis was induced in Swiss albino mice using commercial imiquimod cream (IMQ). Mice were randomly divided into the IMQ untreated control group and the IMQ treated group that was treated with ATSLA twice, on day 0 and day 3. Additional mice served as normal controls. Assessment of skin thickness, erythema, and scales was recorded. Total skin scores were calculated. Skin MDA levels, splenic indices, serum and skin IL-23, and tumor necrosis factor alpha (TNF-α) were measured. Skin sections were stained with H&E and immune stained for CD68-positive cells using immunohistochemistry. Treatment with ATSLA significantly reduced skin thickness, erythema, scales, and total skin scores in the IMQ-treated group compared to the untreated control. This was accompanied by a reduction in the splenic index, skin MDA levels, IL-23, and TNF-α in both the skin and serum of the treated group. Pathologically, skin sections of the treated group showed less epidermal thickness, acanthosis, hyperkeratosis, and CD68 cell count. The study concluded the immunotherapeutic activity of ATSLA in experimental psoriatic skin lesions. This will enrich the psoriasis immunotherapeutic list with novel candidates of parasitic origin.

References

Bruschi F, Gruden-Movesijan A, Pinto B, Ilic N, Sofronic-Milosavlevic L . Trichinella spiralis excretory-secretory products downregulate MMP-9 in Dark Agouti rats affected by experimental autoimmune encephalomyelitis. Exp Parasitol. 2021; 225:108112. DOI: 10.1016/j.exppara.2021.108112. View

Ding J, Liu X, Bai X, Wang Y, Li J, Wang C . inflammation modulator. J Helminthol. 2020; 94:e193. DOI: 10.1017/S0022149X20000802. View

Eissa M, El-Azzouni M, Baddour N, Boulos L . Vaccination trial against experimental trichrinellosis using autoclaved Trichinella spiralis larvae vaccine (ATSLV). J Egypt Soc Parasitol. 2003; 33(1):219-28. View

Eissa M, El-Azzouni M, Mady R, Fathy F, Baddour N . Initial characterization of an autoclaved Toxoplasma vaccine in mice. Exp Parasitol. 2012; 131(3):310-6. DOI: 10.1016/j.exppara.2012.05.001. View

Eissa M, Ismail C, El-Azzouni M, Ghazy A, Hadi M . Immuno-therapeutic potential of Schistosoma mansoni and Trichinella spiralis antigens in a murine model of colon cancer. Invest New Drugs. 2018; 37(1):47-56. DOI: 10.1007/s10637-018-0609-6. View

Eissa M, Gaafar M, Younis L, Ismail C, El Skhawy N . Prophylactic antineoplastic activity of Toxoplasma gondii RH derived antigen against ehrlich solid carcinoma with evidence of shared antigens by comparative immunoblotting. Infect Agent Cancer. 2023; 18(1):21. PMC: 10082516. DOI: 10.1186/s13027-023-00500-3. View

Eissa M, Salem A, El Skhawy N . Parasites revive hope for cancer therapy. Eur J Med Res. 2024; 29(1):489. PMC: 11453045. DOI: 10.1186/s40001-024-02057-2. View

Furue K, Ito T, Furue M . Differential efficacy of biologic treatments targeting the TNF-α/IL-23/IL-17 axis in psoriasis and psoriatic arthritis. Cytokine. 2018; 111:182-188. DOI: 10.1016/j.cyto.2018.08.025. View

Ismail C, Eissa M, Gaafar M, Younis L, El Skhawy N . Toxoplasma gondii-derived antigen modifies tumor microenvironment of Ehrlich solid carcinoma murine model and enhances immunotherapeutic activity of cyclophosphamide. Med Oncol. 2023; 40(5):136. PMC: 10073061. DOI: 10.1007/s12032-023-01994-y. View

10.

Jabeen M, Boisgard A, Danoy A, El Kholti N, Salvi J, Boulieu R . Advanced Characterization of Imiquimod-Induced Psoriasis-Like Mouse Model. Pharmaceutics. 2020; 12(9). PMC: 7558091. DOI: 10.3390/pharmaceutics12090789. View

11.

Jiang Y, Chen Y, Yu Q, Shi Y . Biologic and Small-Molecule Therapies for Moderate-to-Severe Psoriasis: Focus on Psoriasis Comorbidities. BioDrugs. 2023; 37(1):35-55. PMC: 9837020. DOI: 10.1007/s40259-022-00569-z. View

12.

Jin X, Yang Y, Bai X, Shi H, Zhang W, Zhang Z . Dendritic cells treated by Trichinella spiralis muscle larval excretory/secretory products alleviate TNBS-induced colitis in mice. Int Immunopharmacol. 2019; 70:378-386. DOI: 10.1016/j.intimp.2019.02.028. View

13.

Jin X, Bai X, Zhao Y, Dong Z, Pang J, Liu M . Nrf2 Participates in M2 Polarization by to Alleviate TNBS-Induced Colitis in Mice. Front Immunol. 2021; 12:698494. PMC: 8261282. DOI: 10.3389/fimmu.2021.698494. View

14.

Khueangchiangkhwang S, Wu Z, Nagano I, Maekawa Y . -secreted 53 kDa protein ameliorates imiquimod-induced psoriasis by inhibiting the IL-23/IL-17 axis in mice. Biochem Biophys Rep. 2023; 33:101415. PMC: 9813687. DOI: 10.1016/j.bbrep.2022.101415. View

15.

Kierasinska M, Donskow-Lysoniewska K . Both the microbiome and the macrobiome can influence immune responsiveness in psoriasis. Cent Eur J Immunol. 2022; 46(4):502-508. PMC: 8808298. DOI: 10.5114/ceji.2021.110314. View

16.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

17.

Maizels R . Parasitic helminth infections and the control of human allergic and autoimmune disorders. Clin Microbiol Infect. 2016; 22(6):481-6. DOI: 10.1016/j.cmi.2016.04.024. View

18.

Medovic M, Jakovljevic V, Zivkovic V, Jeremic N, Jeremic J, Bolevich S . Psoriasis between Autoimmunity and Oxidative Stress: Changes Induced by Different Therapeutic Approaches. Oxid Med Cell Longev. 2022; 2022:2249834. PMC: 8934232. DOI: 10.1155/2022/2249834. View

19.

Plenkowska J, Gabig-Ciminska M, Mozolewski P . Oxidative Stress as an Important Contributor to the Pathogenesis of Psoriasis. Int J Mol Sci. 2020; 21(17). PMC: 7503883. DOI: 10.3390/ijms21176206. View

20.

Radovic I, Gruden-Movsesijan A, Ilic N, Cvetkovic J, Mojsilovic S, Devic M . Immunomodulatory effects of Trichinella spiralis-derived excretory-secretory antigens. Immunol Res. 2015; 61(3):312-25. DOI: 10.1007/s12026-015-8626-4. View