Current Technologies in Snake Venom Analysis and Applications

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2024 Nov 26

PMID 39591213

Authors

Henrique Roman-Ramos

Paulo Lee Ho

Affiliations

Soon will be listed here.

Abstract

This comprehensive review explores the cutting-edge advancements in snake venom research, focusing on the integration of proteomics, genomics, transcriptomics, and bioinformatics. Highlighting the transformative impact of these technologies, the review delves into the genetic and ecological factors driving venom evolution, the complex molecular composition of venoms, and the regulatory mechanisms underlying toxin production. The application of synthetic biology and multi-omics approaches, collectively known as venomics, has revolutionized the field, providing deeper insights into venom function and its therapeutic potential. Despite significant progress, challenges such as the functional characterization of toxins and the development of cost-effective antivenoms remain. This review also discusses the future directions of venom research, emphasizing the need for interdisciplinary collaborations and new technologies (mRNAs, cryo-electron microscopy for structural determinations of toxin complexes, synthetic biology, and other technologies) to fully harness the biomedical potential of venoms and toxins from snakes and other animals.

References

Junqueira-de-Azevedo I, Ho P . A survey of gene expression and diversity in the venom glands of the pitviper snake Bothrops insularis through the generation of expressed sequence tags (ESTs). Gene. 2002; 299(1-2):279-91. DOI: 10.1016/s0378-1119(02)01080-6. View

Li D, Ji F, Huang C, Jia L . High Expression Achievement of Active and Robust Anti-β2 microglobulin Nanobodies via Hosts Selection. Molecules. 2019; 24(16). PMC: 6720793. DOI: 10.3390/molecules24162860. View

Messadi E . Snake Venom Components as Therapeutic Drugs in Ischemic Heart Disease. Biomolecules. 2023; 13(10). PMC: 10605524. DOI: 10.3390/biom13101539. View

Travers S, Hutter C, Austin C, Donnellan S, Buehler M, Ellison C . VenomCap: An exon-capture probe set for the targeted sequencing of snake venom genes. Mol Ecol Resour. 2024; 24(8):e14020. PMC: 11495845. DOI: 10.1111/1755-0998.14020. View

Gutierrez J, Calvete J, Habib A, Harrison R, Williams D, Warrell D . Snakebite envenoming. Nat Rev Dis Primers. 2017; 3:17063. DOI: 10.1038/nrdp.2017.63. View

Tan N, Wong K, Tan C . Venomics of Naja sputatrix, the Javan spitting cobra: A short neurotoxin-driven venom needing improved antivenom neutralization. J Proteomics. 2017; 157:18-32. DOI: 10.1016/j.jprot.2017.01.018. View

Durban J, Perez A, Sanz L, Gomez A, Bonilla F, Rodriguez S . Integrated "omics" profiling indicates that miRNAs are modulators of the ontogenetic venom composition shift in the Central American rattlesnake, Crotalus simus simus. BMC Genomics. 2013; 14:234. PMC: 3660174. DOI: 10.1186/1471-2164-14-234. View

Ki M, Pack S . Fusion tags to enhance heterologous protein expression. Appl Microbiol Biotechnol. 2020; 104(6):2411-2425. DOI: 10.1007/s00253-020-10402-8. View

Calvete J, Juarez P, Sanz L . Snake venomics. Strategy and applications. J Mass Spectrom. 2007; 42(11):1405-14. DOI: 10.1002/jms.1242. View

10.

Kini R, Doley R . Structure, function and evolution of three-finger toxins: mini proteins with multiple targets. Toxicon. 2010; 56(6):855-67. DOI: 10.1016/j.toxicon.2010.07.010. View

11.

Khalek I, Senji Laxme R, Nguyen Y, Khochare S, Patel R, Woehl J . Synthetic development of a broadly neutralizing antibody against snake venom long-chain α-neurotoxins. Sci Transl Med. 2024; 16(735):eadk1867. DOI: 10.1126/scitranslmed.adk1867. View

12.

Emamipour N, Vossoughi M, Mahboudi F, Golkar M, Fard-Esfahani P . Soluble expression of IGF1 fused to DsbA in SHuffle™ T7 strain: optimization of expression and purification by Box-Behnken design. Appl Microbiol Biotechnol. 2019; 103(8):3393-3406. DOI: 10.1007/s00253-019-09719-w. View

13.

Lechner M, Findeiss S, Steiner L, Marz M, Stadler P, Prohaska S . Proteinortho: detection of (co-)orthologs in large-scale analysis. BMC Bioinformatics. 2011; 12:124. PMC: 3114741. DOI: 10.1186/1471-2105-12-124. View

14.

Perry B, Gopalan S, Pasquesi G, Schield D, Westfall A, Smith C . Snake venom gene expression is coordinated by novel regulatory architecture and the integration of multiple co-opted vertebrate pathways. Genome Res. 2022; 32(6):1058-1073. PMC: 9248877. DOI: 10.1101/gr.276251.121. View

15.

Chavda V, Soni S, Vora L, Soni S, Khadela A, Ajabiya J . mRNA-Based Vaccines and Therapeutics for COVID-19 and Future Pandemics. Vaccines (Basel). 2022; 10(12). PMC: 9785933. DOI: 10.3390/vaccines10122150. View

16.

Krizaj I . Toxinology and Pharmacology of Snake Venoms. Toxins (Basel). 2023; 15(3). PMC: 10051782. DOI: 10.3390/toxins15030212. View

17.

Quiroz S, Henao Castaneda I, Granados J, Patino A, Preciado L, Pereanez J . Inhibitory Effects of Varespladib, CP471474, and Their Potential Synergistic Activity on and Venoms. Molecules. 2022; 27(23). PMC: 9737558. DOI: 10.3390/molecules27238588. View

18.

Hogan M, Holding M, Nystrom G, Colston T, Bartlett D, Mason A . The genetic regulatory architecture and epigenomic basis for age-related changes in rattlesnake venom. Proc Natl Acad Sci U S A. 2024; 121(16):e2313440121. PMC: 11032440. DOI: 10.1073/pnas.2313440121. View

19.

Preciado L, Pereanez J, Azhagiya Singam E, Comer J . Interactions between Triterpenes and a P-I Type Snake Venom Metalloproteinase: Molecular Simulations and Experiments. Toxins (Basel). 2018; 10(10). PMC: 6215199. DOI: 10.3390/toxins10100397. View

20.

Julve Parreno J, Huet E, Fernandez-Del-Carmen A, Segura A, Venturi M, Gandia A . A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins. Plant Biotechnol J. 2017; 16(3):727-736. PMC: 5814581. DOI: 10.1111/pbi.12823. View