Comparison of Microalgae and Lipid Extracts Effects on UVA-Induced Changes in Human Skin Fibroblasts Proteome

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2024 Nov 26

PMID 39590789

Authors

Sinemyiz Atalay Ekiner

Agnieszka Gegotek

Pedro Domingues

Maria Rosario Domingues

Elzbieta Skrzydlewska

Affiliations

Soon will be listed here.

Abstract

Lipid extracts from the microalgae and have great potential to prevent ultraviolet A (UVA)-induced metabolic disorders. Therefore, the aim of this study has been to analyze their cytoprotective effect, focused on maintaining intracellular redox balance and inflammation in UVA-irradiated skin fibroblasts, at the proteome level. The above lipid extracts reversed the suppression of the antioxidant response caused by UVA radiation, which was more visible in the case of . Modulations of interactions between heme oxygenase-1 and matrix metalloproteinase 1/Parkinson's disease protein 7/transcript1-α/β, as well as thioredoxin and migration inhibitory factor/Parkinson's disease protein 7/calnexin/ATPase p97, created key molecular signaling underlying their cytoprotective actions. Moreover, they reduced pro-inflammatory processes in the control group but they also showed the potential to regulate the cellular inflammatory response by changing inflammasome signaling associated with the changes in the caspase-1 interaction area, including heat shock proteins HSP90, HSPA8, and vimentin. Therefore, lipid extracts from and protect skin fibroblast metabolism from UVA-induced damage by restoring the redox balance and regulating inflammatory signaling pathways. Thus, those extracts have proven to have great potential to be used in cosmetic or cosmeceutical products to protect the skin against the effects of solar radiation. However, the possibility of their use requires the evaluation of their effects at the skin level in in vivo and clinical studies.

References

Almanza A, Carlesso A, Chintha C, Creedican S, Doultsinos D, Leuzzi B . Endoplasmic reticulum stress signalling - from basic mechanisms to clinical applications. FEBS J. 2018; 286(2):241-278. PMC: 7379631. DOI: 10.1111/febs.14608. View

Luu Hoang K, Anstee J, Arnold J . The Diverse Roles of Heme Oxygenase-1 in Tumor Progression. Front Immunol. 2021; 12:658315. PMC: 8044534. DOI: 10.3389/fimmu.2021.658315. View

Chua P, Ow S, Ng C, Huang W, Low J, Tan P . Peroxiredoxin 3 regulates breast cancer progression via ERK-mediated MMP-1 expression. Cancer Cell Int. 2024; 24(1):59. PMC: 10845805. DOI: 10.1186/s12935-024-03248-x. View

Zhang Y, Lu S, Fan S, Xu L, Jiang X, Wang K . Macrophage migration inhibitory factor activates the inflammatory response in joint capsule fibroblasts following post-traumatic joint contracture. Aging (Albany NY). 2021; 13(4):5804-5823. PMC: 7950233. DOI: 10.18632/aging.202505. View

Rognoni E, Goss G, Hiratsuka T, Sipila K, Kirk T, Kober K . Role of distinct fibroblast lineages and immune cells in dermal repair following UV radiation-induced tissue damage. Elife. 2021; 10. PMC: 8747514. DOI: 10.7554/eLife.71052. View

Luczaj W, Gegotek A, Conde T, Domingues M, Domingues P, Skrzydlewska E . Lipidomic assessment of the impact of Nannochloropsis oceanica microalga lipid extract on human skin keratinocytes exposed to chronic UVB radiation. Sci Rep. 2023; 13(1):22302. PMC: 10724133. DOI: 10.1038/s41598-023-49827-2. View

Veres-Szekely A, Bernath M, Pap D, Rokonay R, Szebeni B, Takacs I . PARK7 Diminishes Oxidative Stress-Induced Mucosal Damage in Celiac Disease. Oxid Med Cell Longev. 2020; 2020:4787202. PMC: 7492931. DOI: 10.1155/2020/4787202. View

Zhong Q, Xiao X, Qiu Y, Xu Z, Chen C, Chong B . Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications. MedComm (2020). 2023; 4(3):e261. PMC: 10152985. DOI: 10.1002/mco2.261. View

Conde T, Couto D, Melo T, Costa M, Silva J, Domingues M . Polar lipidomic profile shows Chlorococcum amblystomatis as a promising source of value-added lipids. Sci Rep. 2021; 11(1):4355. PMC: 7902829. DOI: 10.1038/s41598-021-83455-y. View

10.

Byrd A, Belkaid Y, Segre J . The human skin microbiome. Nat Rev Microbiol. 2018; 16(3):143-155. DOI: 10.1038/nrmicro.2017.157. View

11.

Espinosa-Diez C, Miguel V, Mennerich D, Kietzmann T, Sanchez-Perez P, Cadenas S . Antioxidant responses and cellular adjustments to oxidative stress. Redox Biol. 2015; 6:183-197. PMC: 4534574. DOI: 10.1016/j.redox.2015.07.008. View

12.

Xue N, Liu Y, Jin J, Ji M, Chen X . Chlorogenic Acid Prevents UVA-Induced Skin Photoaging through Regulating Collagen Metabolism and Apoptosis in Human Dermal Fibroblasts. Int J Mol Sci. 2022; 23(13). PMC: 9266774. DOI: 10.3390/ijms23136941. View

13.

Zhang Z, Wang Y, Li C, Shi Z, Hao Q, Wang W . The Transitional Endoplasmic Reticulum ATPase p97 Regulates the Alternative Nuclear Factor NF-κB Signaling via Partial Degradation of the NF-κB Subunit p100. J Biol Chem. 2015; 290(32):19558-68. PMC: 4528123. DOI: 10.1074/jbc.M114.630061. View

14.

Kong J, Hu X, Cai W, Wang Y, Chi C, Wang B . Bioactive Peptides from Skipjack Tuna Cardiac Arterial Bulbs (II): Protective Function on UVB-Irradiated HaCaT Cells through Antioxidant and Anti-Apoptotic Mechanisms. Mar Drugs. 2023; 21(2). PMC: 9962892. DOI: 10.3390/md21020105. View

15.

Gegotek A, Domingues P, Wronski A, Wojcik P, Skrzydlewska E . Proteomic plasma profile of psoriatic patients. J Pharm Biomed Anal. 2018; 155:185-193. DOI: 10.1016/j.jpba.2018.03.068. View

16.

Forman H, Zhang H . Targeting oxidative stress in disease: promise and limitations of antioxidant therapy. Nat Rev Drug Discov. 2021; 20(9):689-709. PMC: 8243062. DOI: 10.1038/s41573-021-00233-1. View

17.

DOrazio J, Jarrett S, Amaro-Ortiz A, Scott T . UV radiation and the skin. Int J Mol Sci. 2013; 14(6):12222-48. PMC: 3709783. DOI: 10.3390/ijms140612222. View

18.

Stasiewicz A, Conde T, Domingues M, Domingues P, Biernacki M, Skrzydlewska E . Comparison of the Regenerative Metabolic Efficiency of Lipid Extracts from Microalgae and on Fibroblasts. Antioxidants (Basel). 2024; 13(3). PMC: 10967502. DOI: 10.3390/antiox13030276. View

19.

Chen K, Rao Z, Dong S, Chen Y, Wang X, Luo Y . Roles of the fibroblast growth factor signal transduction system in tissue injury repair. Burns Trauma. 2022; 10:tkac005. PMC: 8946634. DOI: 10.1093/burnst/tkac005. View

20.

Choi J, Hwang C, Lee H, Kim H, Han S, Hong J . Inhibitory effect of ethanol extract of Nannochloropsis oceanica on lipopolysaccharide-induced neuroinflammation, oxidative stress, amyloidogenesis and memory impairment. Oncotarget. 2017; 8(28):45517-45530. PMC: 5542205. DOI: 10.18632/oncotarget.17268. View