An Algerian Soil-Living Strain As Source of Antifungal Compounds for the Management of the Pea Pathogen F. Sp.

Overview

Journal J Fungi (Basel)

Date 2024 Nov 26

PMID 39590702

Authors

Marco Masi

Dorsaf Nedjar

Moustafa Bani

Ivana Staiano

Maria Michela Salvatore

Karima Khenaka

Stefany Castaldi

Jesus Garcia Zorrilla

Anna Andolfi

Rachele Isticato

Alessio Cimmino

Affiliations

Soon will be listed here.

Abstract

Fusarium wilt caused by f. sp. () poses significant threats to pea cultivation worldwide. Controlling this disease is mainly achieved through the integration of various disease management procedures, among which biological control has proven to be a safe and effective approach. This study aims to extract and identify antifungal secondary metabolites from KRO3 strain and assess their effectiveness in inhibiting the in vitro growth of . This bacterial strain exerts in vitro antagonistic activity against , achieving highly significant inhibition over one week. The ethyl acetate extract, obtained from its ISP2 agar medium culture, also exhibited strong antifungal activity, maintaining an inhibition rate of approximately 90% at concentrations up to 250 µg/plug compared to the control. Thus, the organic extract has been fractionated using chromatographic techniques and its bioguided purification allowed us to isolate the main bioactive compound. This latter was identified as metacycloprodigiosin using nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and specific optical rotation data. Metacycloprodigiosin demonstrates dose-dependent inhibitory activity against the phytopathogen with an effective concentration of 125 µg/plug. The other secondary metabolites present in the ethyl acetate extract were also identified by gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). This study highlighted the potential of KRO3 strain and its antimicrobial compounds for the management of the pea pathogen f. sp. .

Citing Articles

Inhibitory Effect and Mechanism of Dryocrassin ABBA Against .

Wang W, Zhang D, Thomsen P, Sun M, Chang Y Int J Mol Sci. 2025; 26(4).

PMID: 40004037 PMC: 11855913. DOI: 10.3390/ijms26041573.

References

Kimata S, Izawa M, Kawasaki T, Hayakawa Y . Identification of a prodigiosin cyclization gene in the roseophilin producer and production of a new cyclized prodigiosin in a heterologous host. J Antibiot (Tokyo). 2016; 70(2):196-199. DOI: 10.1038/ja.2016.94. View

Peixoto D, Ferreira E, Lourenco A, Johnson J, Lobo A, Polavarapu P . (R)-Metacycloprodigiosin-HCl: Chiroptical properties and structure. Chirality. 2018; 30(7):932-942. DOI: 10.1002/chir.22867. View

Yun T, Feng R, Zhou D, Pan Y, Chen Y, Wang F . Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1-14 from cassava rhizosphere. PLoS One. 2018; 13(11):e0206497. PMC: 6241123. DOI: 10.1371/journal.pone.0206497. View

Torres-Rodriguez J, Reyes-Perez J, Quinones-Aguilar E, Hernandez-Montiel L . Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications. Plants (Basel). 2022; 11(23). PMC: 9736024. DOI: 10.3390/plants11233201. View

Abbasi S, Safaie N, Sadeghi A, Shamsbakhsh M . Strains Induce Resistance to f. sp. Race 3 in Tomato Through Different Molecular Mechanisms. Front Microbiol. 2019; 10:1505. PMC: 6616268. DOI: 10.3389/fmicb.2019.01505. View

Wei Y, Zhao Y, Zhou D, Qi D, Li K, Tang W . A Newly Isolated sp. YYS-7 With a Broad-Spectrum Antifungal Activity Improves the Banana Plant Resistance to f. sp. Tropical Race 4. Front Microbiol. 2020; 11:1712. PMC: 7438861. DOI: 10.3389/fmicb.2020.01712. View

Zdorovenko E, Dmitrenok A, Masi M, Castaldi S, Muzio F, Isticato R . Structural studies on the O-specific polysaccharide of the lipopolysaccharide from Pseudomonas donghuensis strain SVBP6, with antifungal activity against the phytopathogenic fungus Macrophomina phaseolina. Int J Biol Macromol. 2021; 182:2019-2023. DOI: 10.1016/j.ijbiomac.2021.05.187. View

Lal D, Dev D, Kumari S, Pandey S, Aparna , Sharma N . Fusarium wilt pandemic: current understanding and molecular perspectives. Funct Integr Genomics. 2024; 24(2):41. DOI: 10.1007/s10142-024-01319-w. View

Habash S, Brass H, Klein A, Klebl D, Weber T, Classen T . Novel Prodiginine Derivatives Demonstrate Bioactivities on Plants, Nematodes, and Fungi. Front Plant Sci. 2020; 11:579807. PMC: 7596250. DOI: 10.3389/fpls.2020.579807. View

10.

Girao M, Ribeiro I, Ribeiro T, Azevedo I, Pereira F, Urbatzka R . Actinobacteria Isolated From : A Source of New Bioactive Compounds. Front Microbiol. 2019; 10:683. PMC: 6465344. DOI: 10.3389/fmicb.2019.00683. View

11.

Yoon S, Ha S, Kwon S, Lim J, Kim Y, Seo H . Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol. 2016; 67(5):1613-1617. PMC: 5563544. DOI: 10.1099/ijsem.0.001755. View

12.

Paul T, Mondal A, Bandyopadhyay T, Mahata N, Bhunia B . Downstream Process Development for Extraction of Prodigiosin: Statistical Optimization, Kinetics, and Biochemical Characterization. Appl Biochem Biotechnol. 2022; 194(11):5403-5418. DOI: 10.1007/s12010-022-04053-w. View

13.

Hu D, Clift M, Lazarski K, Thomson R . Enantioselective total synthesis and confirmation of the absolute and relative stereochemistry of streptorubin B. J Am Chem Soc. 2010; 133(6):1799-804. PMC: 3073088. DOI: 10.1021/ja109165f. View

14.

Rahul S, Chandrashekhar P, Hemant B, Chandrakant N, Laxmikant S, Satish P . Nematicidal activity of microbial pigment from Serratia marcescens. Nat Prod Res. 2014; 28(17):1399-404. DOI: 10.1080/14786419.2014.904310. View

15.

Araujo R, Zavala N, Castillo-Zacarias C, Barocio M, Hidalgo-Vazquez E, Parra-Arroyo L . Recent Advances in Prodigiosin as a Bioactive Compound in Nanocomposite Applications. Molecules. 2022; 27(15). PMC: 9370345. DOI: 10.3390/molecules27154982. View

16.

WASSERMAN H, Rodgers G, Keith D . Metacycloprodigiosin, a tripyrrole pigment from Streptomyces longisporus ruber. J Am Chem Soc. 1969; 91(5):1263-4. DOI: 10.1021/ja01033a065. View

17.

Leulmi N, Sighel D, Defant A, Khenaka K, Boulahrouf A, Mancini I . Nigericin and grisorixin methyl ester from the Algerian soil-living Streptomyces youssoufiensis SF10 strain: a computational study on their epimeric structures and evaluation of glioblastoma stem cells growth inhibition. Nat Prod Res. 2018; 33(2):266-273. DOI: 10.1080/14786419.2018.1446014. View

18.

Qi D, Zou L, Zhou D, Zhang M, Wei Y, Zhang L . Identification and Antifungal Mechanism of a Novel Actinobacterium sp. nov. Against f. sp. Tropical Race 4 of Banana. Front Microbiol. 2021; 12:722661. PMC: 8600237. DOI: 10.3389/fmicb.2021.722661. View

19.

Ilic-Tomic T, Gencic M, Zivkovic M, Vasiljevic B, Djokic L, Nikodinovic-Runic J . Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp. NP10. Appl Microbiol Biotechnol. 2015; 99(11):4815-33. DOI: 10.1007/s00253-014-6364-5. View

20.

Navas-Cortes J, Hau B, Jimenez-Diaz R . Effect of Sowing Date, Host Cultivar, and Race of Fusarium oxysporum f. sp. ciceris on Development of Fusarium Wilt of Chickpea. Phytopathology. 2008; 88(12):1338-46. DOI: 10.1094/PHYTO.1998.88.12.1338. View