Correlation Between Female Pronuclear/cytoplasmic Ratio and Number of Chromosomes in Mouse Zygotic Stage: Implications for Aneuploidy Assessment in ART

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2024 Nov 25

PMID 39585518

Authors

Wei Xiao

Sakura Akao

Junko Otsuki

Affiliations

Soon will be listed here.

Abstract

Purpose: The objective of this study was to investigate the correlation between the pronuclear/cytoplasmic (PN/C) ratio and the number of chromosomes in mouse zygotes to understand the implications of pronuclear size regulation in early embryonic development.

Methods: A combination of enucleation and aggregated chromosomes/chromatin (AC) transfer was utilized to create oocytes with varying numbers of chromosomes. Time-lapse imaging and immunofluorescence staining were employed to analyze pronuclear dynamics and chromosomal configurations.

Results: Higher chromosome numbers correspond to a larger PN/C ratio. Oocytes with a higher number of chromosomes exhibited larger pronuclei.

Conclusion: The study underscores the complexity of pronuclear size regulation and its correlation with the number of chromosomes. The findings suggest potential applications in ART, where assessing the PN/C ratio could serve as a biomarker for zygote quality and aneuploidy.

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