Multi-omics Analysis of Klebsiella Pneumoniae Revealed Opposing Effects of Rutin and Luteolin on Strain Growth

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2024 Nov 25

PMID 39585437

Authors

Zhibin Wang

Wanxia Shen

Yuejiao Li

Xiaoyun Wang

Xiaolin Zhong

Xing Wang

Affiliations

Soon will be listed here.

Abstract

The emergence of pathogenic bacteria resistant to conventional antibiotics is becoming increasingly challenging. Plant-derived flavonoids are potential alternatives to antibiotics, owing to their antimicrobial properties. However, the molecular mechanisms through which they inhibit the growth of pathogenic microorganisms remain unclear. Therefore, Klebsiella pneumoniae ATCC700603 was separately incubated in two flavonoids to elucidate their inhibitory mechanism. Metabolomic and transcriptomic analyses were performed after 4-h incubation. In total, 5483 genes and 882 metabolites were identified. Compared to the untreated control, rutin and luteolin activated 507 and 374 differentially expressed genes (DEGs), respectively. However, the number of differential abundant metabolites (DAMs) remained the same. The top 10 correlated DEGs and DAMs were identified within each comparative group after a correlation analysis. Rutin induced the accumulation of unique metabolites and suppressed gene expression whereas luteolin did not. Our results explain the disparate effects of these two flavonoids and demonstrate the inhibitory mechanism of rutin on strain growth.

References

Kupnik K, Primozic M, Vasic K, Knez Z, Leitgeb M . A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate ( L.) Peels and Seeds. Plants (Basel). 2021; 10(8). PMC: 8402121. DOI: 10.3390/plants10081554. View

Fu Y, Yang Z, Cai Y, Liu H, Li S, Kou N . Effect of Bloodletting at Shaoshang and Shangyang Acupuncture Points on Outcome and Prognosis of Severe Community-Acquired Pneumonia in the Elderly. Dis Markers. 2021; 2021:3295021. PMC: 8563111. DOI: 10.1155/2021/3295021. View

Wang P, Jiang S, Li Y, Luo Q, Lin J, Hu L . Virus-like mesoporous silica-coated plasmonic Ag nanocube with strong bacteria adhesion for diabetic wound ulcer healing. Nanomedicine. 2021; 34:102381. DOI: 10.1016/j.nano.2021.102381. View

Escobar B, Montenegro I, Villena J, Werner E, Godoy P, Olguin Y . Hemi-Synthesis and Anti-Oomycete Activity of Analogues of Isocordoin. Molecules. 2017; 22(6). PMC: 6152731. DOI: 10.3390/molecules22060968. View

Singh V, Chibale K . Strategies to Combat Multi-Drug Resistance in Tuberculosis. Acc Chem Res. 2021; 54(10):2361-2376. PMC: 8154215. DOI: 10.1021/acs.accounts.0c00878. View

Lin S, Wade J, Liu S . Design of Flavonoid-Based Mimetics of Cationic Antimicrobial Peptides: Discovery, Development, and Applications. Acc Chem Res. 2020; 54(1):104-119. DOI: 10.1021/acs.accounts.0c00550. View

Wang Z, Yu Q, Shen W, El Mohtar C, Zhao X, Gmitter Jr F . Functional study of CHS gene family members in citrus revealed a novel CHS gene affecting the production of flavonoids. BMC Plant Biol. 2018; 18(1):189. PMC: 6134715. DOI: 10.1186/s12870-018-1418-y. View

Sheng Z, Jiang Y, Liu J, Yang B . UHPLC-MS/MS Analysis on Flavonoids Composition in and Their Antioxidant Activity. Antioxidants (Basel). 2021; 10(11). PMC: 8614773. DOI: 10.3390/antiox10111852. View

Li G, Ding K, Qiao Y, Zhang L, Zheng L, Pan T . Flavonoids Regulate Inflammation and Oxidative Stress in Cancer. Molecules. 2020; 25(23). PMC: 7729519. DOI: 10.3390/molecules25235628. View

10.

HARBORNE J, Williams C . Advances in flavonoid research since 1992. Phytochemistry. 2000; 55(6):481-504. DOI: 10.1016/s0031-9422(00)00235-1. View

11.

Cushnie T, Lamb A . Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005; 26(5):343-56. PMC: 7127073. DOI: 10.1016/j.ijantimicag.2005.09.002. View

12.

Farhadi F, Khameneh B, Iranshahi M, Iranshahy M . Antibacterial activity of flavonoids and their structure-activity relationship: An update review. Phytother Res. 2018; 33(1):13-40. DOI: 10.1002/ptr.6208. View

13.

Benevides Bahiense J, Marques F, Figueira M, Vargas T, Kondratyuk T, Endringer D . Potential anti-inflammatory, antioxidant and antimicrobial activities of Sambucus australis. Pharm Biol. 2017; 55(1):991-997. PMC: 6130686. DOI: 10.1080/13880209.2017.1285324. View

14.

Duda-Madej A, Kozlowska J, Krzyzek P, Aniol M, Seniuk A, Jermakow K . Antimicrobial -Alkyl Derivatives of Naringenin and Their Oximes Against Multidrug-Resistant Bacteria. Molecules. 2020; 25(16). PMC: 7464300. DOI: 10.3390/molecules25163642. View

15.

Xiao Z, Wang X, Wang P, Zhou Y, Zhang J, Zhang L . Design, synthesis, and evaluation of novel fluoroquinolone-flavonoid hybrids as potent antibiotics against drug-resistant microorganisms. Eur J Med Chem. 2014; 80:92-100. DOI: 10.1016/j.ejmech.2014.04.037. View

16.

Eumkeb G, Chukrathok S . Synergistic activity and mechanism of action of ceftazidime and apigenin combination against ceftazidime-resistant Enterobacter cloacae. Phytomedicine. 2012; 20(3-4):262-9. DOI: 10.1016/j.phymed.2012.10.008. View

17.

Lo Giudice A, Bruni V, Michaud L . Characterization of Antarctic psychrotrophic bacteria with antibacterial activities against terrestrial microorganisms. J Basic Microbiol. 2007; 47(6):496-505. DOI: 10.1002/jobm.200700227. View

18.

Wallace J, Bowlin N, Mills D, Saenkham P, Kwasny S, Opperman T . Discovery of bacterial fatty acid synthase type II inhibitors using a novel cellular bioluminescent reporter assay. Antimicrob Agents Chemother. 2015; 59(9):5775-87. PMC: 4538483. DOI: 10.1128/AAC.00686-15. View

19.

Rutschlin S, Bottcher T . Inhibitors of Bacterial Swarming Behavior. Chemistry. 2019; 26(5):964-979. PMC: 7027876. DOI: 10.1002/chem.201901961. View

20.

Lee J, Regmi S, Kim J, Cho M, Yun H, Lee C . Apple flavonoid phloretin inhibits Escherichia coli O157:H7 biofilm formation and ameliorates colon inflammation in rats. Infect Immun. 2011; 79(12):4819-27. PMC: 3232668. DOI: 10.1128/IAI.05580-11. View