Enhancing Skin Regeneration Efficacy of Human Dermal Fibroblasts Using Carboxymethyl Cellulose-Coated Biodegradable Polymer

Overview

Journal Tissue Eng Regen Med

Date 2024 Nov 23

PMID 39579169

Authors

You Bin Lee

Dong-Hyun Lee

Youn Chul Kim

Suk Ho Bhang

Affiliations

Soon will be listed here.

Abstract

Background: Polylactic acid (PLA) is extensively used in the medical and cosmetic industries for skin regeneration and as a dermal filler due to its biocompatibility and biodegradability. However, the effectiveness of PLA as a cosmetic filler is limited by its slow degradation rate and poor cell attachment properties. Recent studies have focused on enhancing the performance of PLA by combining it with other materials. This study aimed to evaluate the performance of carboxymethyl cellulose (CMC), known for its high biocompatibility, in comparison with the widely used hyaluronic acid (HA).

Methods: Two types of PLA-based particles, HA-PLA and CMC-PLA were synthesized by combining PLA with HA and CMC, respectively. After characterizing the particles, we evaluated cell adhesion and viability using human dermal fibroblasts and analyzed gene and protein expression related to cell attachment and angiogenic paracrine factors.

Results: The CMC-PLA particles maintained a more uniform size distribution than the HA-PLA particles and exhibited superior cell adhesion properties. Cells attached on the CMC-PLA particles showed enhanced secretion of angiogenic paracrine factors, suggesting a potential improvement in therapeutic efficacy.

Conclusion: CMC-PLA particles demonstrated superior cell adhesion and secretion capabilities compared with HA-PLA particles, indicating their potential for application in skin regeneration and tissue recovery. Further research, including in vivo studies, is required to fully explore and validate the therapeutic potential of CMC-PLA particles.

References

Abu Hajleh M, Al-Samydai A, Al-Dujaili E . Nano, micro particulate and cosmetic delivery systems of polylactic acid: A mini review. J Cosmet Dermatol. 2020; 19(11):2805-2811. DOI: 10.1111/jocd.13696. View

Bartus C, William Hanke C, Daro-Kaftan E . A decade of experience with injectable poly-L-lactic acid: a focus on safety. Dermatol Surg. 2013; 39(5):698-705. DOI: 10.1111/dsu.12128. View

Ao Y, Yi Y, Wu G . Application of PLLA (Poly-L-Lactic acid) for rejuvenation and reproduction of facial cutaneous tissue in aesthetics: A review. Medicine (Baltimore). 2024; 103(11):e37506. PMC: 10939544. DOI: 10.1097/MD.0000000000037506. View

Zou Y, Cao M, Tao L, Wu S, Zhou H, Zhang Y . Lactate triggers KAT8-mediated LTBP1 lactylation at lysine 752 to promote skin rejuvenation by inducing collagen synthesis in fibroblasts. Int J Biol Macromol. 2024; 277(Pt 3):134482. DOI: 10.1016/j.ijbiomac.2024.134482. View

Zhu A, Zhang M, Wu J, Shen J . Covalent immobilization of chitosan/heparin complex with a photosensitive hetero-bifunctional crosslinking reagent on PLA surface. Biomaterials. 2002; 23(23):4657-65. DOI: 10.1016/s0142-9612(02)00215-6. View

Rahmany M, Van Dyke M . Biomimetic approaches to modulate cellular adhesion in biomaterials: A review. Acta Biomater. 2012; 9(3):5431-7. DOI: 10.1016/j.actbio.2012.11.019. View

Anderson J, Rodriguez A, Chang D . Foreign body reaction to biomaterials. Semin Immunol. 2007; 20(2):86-100. PMC: 2327202. DOI: 10.1016/j.smim.2007.11.004. View

Schroepfer M, Junghans F, Voigt D, Meyer M, Breier A, Schulze-Tanzil G . Gas-Phase Fluorination on PLA Improves Cell Adhesion and Spreading. ACS Omega. 2020; 5(10):5498-5507. PMC: 7081643. DOI: 10.1021/acsomega.0c00126. View

Yao Q, Cosme J, Xu T, Miszuk J, Picciani P, Fong H . Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation. Biomaterials. 2016; 115:115-127. PMC: 5181114. DOI: 10.1016/j.biomaterials.2016.11.018. View

10.

Lin S, Christen M . Polycaprolactone-based dermal filler complications: A retrospective study of 1111 treatments. J Cosmet Dermatol. 2020; 19(8):1907-1914. PMC: 7497126. DOI: 10.1111/jocd.13518. View

11.

Weng L, Rostamzadeh P, Nooryshokry N, Le H, Golzarian J . In vitro and in vivo evaluation of biodegradable embolic microspheres with tunable anticancer drug release. Acta Biomater. 2013; 9(6):6823-33. DOI: 10.1016/j.actbio.2013.02.017. View

12.

Park S, Yu Y, Park G, Shin S, Kim J, Lee B . Proliferation-Related Features of the Human Mesenchymal Stem Cells Derived from Palatine Tonsils, Adipose Tissues, and Bone Marrow. Tissue Eng Regen Med. 2023; 20(7):1119-1132. PMC: 10645842. DOI: 10.1007/s13770-023-00564-7. View

13.

Majhy B, Priyadarshini P, Sen A . Effect of surface energy and roughness on cell adhesion and growth - facile surface modification for enhanced cell culture. RSC Adv. 2022; 11(25):15467-15476. PMC: 8698786. DOI: 10.1039/d1ra02402g. View

14.

Chiarugi P, Giannoni E . Anoikis: a necessary death program for anchorage-dependent cells. Biochem Pharmacol. 2008; 76(11):1352-64. DOI: 10.1016/j.bcp.2008.07.023. View

15.

Lee S, Choi E, Cha M, Hwang K . Cell adhesion and long-term survival of transplanted mesenchymal stem cells: a prerequisite for cell therapy. Oxid Med Cell Longev. 2015; 2015:632902. PMC: 4333334. DOI: 10.1155/2015/632902. View

16.

Bacakova L, Filova E, Parizek M, Ruml T, Svorcik V . Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants. Biotechnol Adv. 2011; 29(6):739-67. DOI: 10.1016/j.biotechadv.2011.06.004. View