Purification and Characterization of Glucose Oxidase from Ligninolytic Cultures of Phanerochaete Chrysosporium

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1986 Apr 1

PMID 3957868

Citations 18

Authors

R L Kelley

C A Reddy

Affiliations

Soon will be listed here.

Abstract

Glucose oxidase, an important source of hydrogen peroxide in lignin-degrading cultures of Phanerochaete chrysosporium, was purified to electrophoretic homogeneity by a combination of ion-exchange and molecular sieve chromatography. The enzyme is a flavoprotein with an apparent native molecular weight of 180,000 and a denatured molecular weight of 80,000. This enzyme does not appear to be a glycoprotein. It gives optimal activity with D-glucose, which is stoichiometrically oxidized to D-gluconate. The enzyme has a relatively broad pH optimum of 4 to 5. It is inhibited by Ag+ (10 mM) and o-phthalate (100 mM), but not by Cu2+, NaF, or KCN (each 10 mM).

Citing Articles

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass.

Bissaro B, Varnai A, Rohr A, Eijsink V Microbiol Mol Biol Rev. 2018; 82(4).

PMID: 30257993 PMC: 6298611. DOI: 10.1128/MMBR.00029-18.

Stimulation of Pyruvate Kinase in the Presence of Glucose to Cope With HO Stress Generated by Its Competitors.

Kim H, Yoon C, Ham H, Seok Y, Park Y Front Microbiol. 2018; 9:1112.

PMID: 29896177 PMC: 5987630. DOI: 10.3389/fmicb.2018.01112.

Bioconversion of lignocellulose materials.

Pothiraj C, Kanmani P, Balaji P Mycobiology. 2013; 34(4):159-65.

PMID: 24039492 PMC: 3769567. DOI: 10.4489/MYCO.2006.34.4.159.

Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green.

Son Y, Kim H, Thiyagarajan S, Xu J, Park S Mycobiology. 2013; 40(4):258-62.

PMID: 23323052 PMC: 3538974. DOI: 10.5941/MYCO.2012.40.4.258.

P450 monooxygenases (P450ome) of the model white rot fungus Phanerochaete chrysosporium.

Syed K, Yadav J Crit Rev Microbiol. 2012; 38(4):339-63.

PMID: 22624627 PMC: 3567848. DOI: 10.3109/1040841X.2012.682050.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Faison B, Kirk T . Relationship Between Lignin Degradation and Production of Reduced Oxygen Species by Phanerochaete chrysosporium. Appl Environ Microbiol. 1983; 46(5):1140-5. PMC: 239531. DOI: 10.1128/aem.46.5.1140-1145.1983. View

Yamada Y, Lizuka K, Aida K, Uemura T . Enzymatic studies on the oxidation of sugar and sugar alcohol. 3. Purification and properties of L-sorbose oxidase from Trametes sanguinea. J Biochem. 1967; 62(2):223-9. DOI: 10.1093/oxfordjournals.jbchem.a128652. View

Janssen F, RUELIUS H . Carbohydrate oxidase, a novel enzyme from Polyporus obtusus. II. Specificity and characterization of reaction products. Biochim Biophys Acta. 1968; 167(3):501-10. DOI: 10.1016/0005-2744(68)90040-5. View

Forney L, Reddy C, Pankratz H . Ultrastructural Localization of Hydrogen Peroxide Production in Ligninolytic Phanerochaete chrysosporium Cells. Appl Environ Microbiol. 1982; 44(3):732-6. PMC: 242083. DOI: 10.1128/aem.44.3.732-736.1982. View

Greene R, Gould J . Fatty acyl-coenzyme A oxidase activity and H2O2 production in Phanerochaete chrysosporium mycelia. Biochem Biophys Res Commun. 1984; 118(2):437-43. DOI: 10.1016/0006-291x(84)91322-6. View

Eckhardt A, Hayes C, Goldstein I . A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels. Anal Biochem. 1976; 73(1):192-7. DOI: 10.1016/0003-2697(76)90154-8. View

Tien M, Kirk T . Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H(2)O(2)-requiring oxygenase. Proc Natl Acad Sci U S A. 1984; 81(8):2280-4. PMC: 345042. DOI: 10.1073/pnas.81.8.2280. View

Robinson J, Cooper J . Method of determining oxygen concentrations in biological media, suitable for calibration of the oxygen electrode. Anal Biochem. 1970; 33(2):390-9. DOI: 10.1016/0003-2697(70)90310-6. View

10.

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View

11.

Andersson L, Renganathan V, Chiu A, Loehr T, Gold M . Spectral characterization of diarylpropane oxygenase, a novel peroxide-dependent, lignin-degrading heme enzyme. J Biol Chem. 1985; 260(10):6080-7. View

12.

Kersten P, Tien M, Kalyanaraman B, Kirk T . The ligninase of Phanerochaete chrysosporium generates cation radicals from methoxybenzenes. J Biol Chem. 1985; 260(5):2609-12. View

13.

SWOBODA B, MASSEY V . PURIFICATION AND PROPERTIES OF THE GLUCOSE OXIDASE FROM ASPERGILLUS NIGER. J Biol Chem. 1965; 240:2209-15. View

14.

CERLETTI P, Strom R, Giordano M . Flavine peptides in tissues: the prosthetic group of succinic dehydrogenase. Arch Biochem Biophys. 1963; 101:423-8. DOI: 10.1016/0003-9861(63)90497-1. View

15.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

16.

Kelley R, Reddy C . Ethylene production from alpha-oxo-gamma-methylthiobutyric acid is a sensitive measure of ligninolytic activity by Phanerochaete chrysosporium. Biochem J. 1982; 206(2):423-5. PMC: 1158601. DOI: 10.1042/bj2060423. View

17.

Kutsuki H, Gold M . Generation of hydroxyl radical and its involvement in lignin degradation by Phanerochaete chrysosporium. Biochem Biophys Res Commun. 1982; 109(2):320-7. DOI: 10.1016/0006-291x(82)91723-5. View

18.

ADAMS Jr E, MAST R, FREE A . Specificity of glucose oxidase. Arch Biochem Biophys. 1960; 91:230-4. DOI: 10.1016/0003-9861(60)90495-1. View