Inactivation of Deposited Bioaerosols on Food Contact Surfaces with UV-C Light Emitting Diode Devices

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2024 Nov 21

PMID 39570036

Authors

Aakash Sharma

Amritpal Singh

Brahmaiah Pendyala

Sampathkumar Balamurugan

Ankit Patras

Affiliations

Soon will be listed here.

Abstract

The airborne transmission of infectious diseases and bioaerosol-induced cross-contamination pose significant challenges in the food, dairy, and pharma industries. This study evaluated the effectiveness of 279 nm UV-C LED irradiation for decontaminating bioaerosols, specifically containing microorganisms such as (C3040- Kanamycin resistant), Enteritidis (ATCC 4931), and (ATCC 4973), on food contact surfaces. Borosilicate glass, silicon rubber, and stainless steel (316L) surfaces were selected for experimentation for their usage in the food industry. A 50 µL cell suspension was aerosolized at 25 psi pressure using a 4-jet BLAM Nebulizer within a customized glass chamber and then deposited onto the surface of the coupons. The serial dilution approach was used for the microbial enumeration, followed by duplicate plating. With a low Root Mean Square Error (RMSE) and high values, the biphasic kinetic model for UV-C inactivation curves of all three pathogens demonstrated the excellent goodness of fit parameters. At a UV-C dose of 6 mJ cm, glass surfaces showed the maximum microbial inactivation (i.e., 2.80, 3.81, and 3.56 log CFU/mL for , , and , respectively). Stainless steel and silicon rubber surfaces showed significant microbial inactivation, but log reductions observed were consistently lower than glass surface. Our research indicates that UV-C LEDs (279 nm) can effectively disinfect bioaerosols on food contact surfaces.IMPORTANCEFood safety is a major public health concern, with contaminated food causing serious illnesses. UV-C light, used for germicidal action, is effective in disinfecting surfaces and is not subject to the same strict legal restrictions as chemical disinfectants, simplifying compliance with food safety regulations. In this study, we evaluated the efficacy of UV-C (279 nm) LED systems for inactivation of surface-deposited bioaerosols of kanamycin-resistant (C3040), Enteritidis (ATCC 4931), and (ATCC 4973). The research outcomes can be used to develop UV-based surface disinfection systems to minimize the risk of foodborne illnesses and enhance safety in high-traffic food preparation areas.

References

Calle A, Fernandez M, Montoya B, Schmidt M, Thompson J . UV-C LED Irradiation Reduces on Chicken and Food Contact Surfaces. Foods. 2021; 10(7). PMC: 8305569. DOI: 10.3390/foods10071459. View

Nyangaresi P, Qin Y, Chen G, Zhang B, Lu Y, Shen L . Effects of single and combined UV-LEDs on inactivation and subsequent reactivation of E. coli in water disinfection. Water Res. 2018; 147:331-341. DOI: 10.1016/j.watres.2018.10.014. View

Nychas G, Skandamis P, Tassou C, Koutsoumanis K . Meat spoilage during distribution. Meat Sci. 2011; 78(1-2):77-89. DOI: 10.1016/j.meatsci.2007.06.020. View

Kim D, Kang D . UVC LED Irradiation Effectively Inactivates Aerosolized Viruses, Bacteria, and Fungi in a Chamber-Type Air Disinfection System. Appl Environ Microbiol. 2018; 84(17). PMC: 6102977. DOI: 10.1128/AEM.00944-18. View

Ramos C, Roque J, Sarmiento D, Suarez L, Sunio J, Tabungar K . Use of ultraviolet-C in environmental sterilization in hospitals: A systematic review on efficacy and safety. Int J Health Sci (Qassim). 2020; 14(6):52-65. PMC: 7644456. View

Kim S, Kim D, Kang D . Using UVC Light-Emitting Diodes at Wavelengths of 266 to 279 Nanometers To Inactivate Foodborne Pathogens and Pasteurize Sliced Cheese. Appl Environ Microbiol. 2015; 82(1):11-7. PMC: 4702654. DOI: 10.1128/AEM.02092-15. View

Li M, Qi J, Zhang H, Huang S, Li L, Gao D . Concentration and size distribution of bioaerosols in an outdoor environment in the Qingdao coastal region. Sci Total Environ. 2011; 409(19):3812-9. DOI: 10.1016/j.scitotenv.2011.06.001. View

Haddrell A, Davies J, Reid J . Dynamics of Particle Size on Inhalation of Environmental Aerosol and Impact on Deposition Fraction. Environ Sci Technol. 2015; 49(24):14512-21. DOI: 10.1021/acs.est.5b01930. View

Kim D, Kim S, Kang D . Bactericidal effect of 266 to 279nm wavelength UVC-LEDs for inactivation of Gram positive and Gram negative foodborne pathogenic bacteria and yeasts. Food Res Int. 2017; 97:280-287. DOI: 10.1016/j.foodres.2017.04.009. View

10.

White J, Nielsen J, Larsen C, Madsen A . Impact of dust on airborne Staphylococcus aureus' viability, culturability, inflammogenicity, and biofilm forming capacity. Int J Hyg Environ Health. 2020; 230:113608. DOI: 10.1016/j.ijheh.2020.113608. View

11.

Schobel H, Diem G, Kiechl J, Chiste D, Bertacchi G, Mayr A . Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology. J Hosp Infect. 2023; 135:11-17. PMC: 10041887. DOI: 10.1016/j.jhin.2022.12.023. View

12.

Marshall K, Tewell M, Tecle S, Leeper M, Sinatra J, Kissler B . Protracted Outbreak of Salmonella Newport Infections Linked to Ground Beef: Possible Role of Dairy Cows - 21 States, 2016-2017. MMWR Morb Mortal Wkly Rep. 2018; 67(15):443-446. PMC: 6191100. DOI: 10.15585/mmwr.mm6715a2. View

13.

Rizzo L, Fiorentino A, Anselmo A . Advanced treatment of urban wastewater by UV radiation: Effect on antibiotics and antibiotic-resistant E. coli strains. Chemosphere. 2013; 92(2):171-6. DOI: 10.1016/j.chemosphere.2013.03.021. View

14.

Hijnen W, Beerendonk E, Medema G . Inactivation credit of UV radiation for viruses, bacteria and protozoan (oo)cysts in water: a review. Water Res. 2006; 40(1):3-22. DOI: 10.1016/j.watres.2005.10.030. View

15.

Bhullar M, Patras A, Kilanzo-Nthenge A, Pokharel B, Yannam S, Rakariyatham K . Microbial inactivation and cytotoxicity evaluation of UV irradiated coconut water in a novel continuous flow spiral reactor. Food Res Int. 2018; 103:59-67. DOI: 10.1016/j.foodres.2017.10.004. View

16.

Bhullar M, Patras A, Kilonzo-Nthenge A, Pokharel B, Sasges M . Ultraviolet inactivation of bacteria and model viruses in coconut water using a collimated beam system. Food Sci Technol Int. 2019; 25(7):562-572. DOI: 10.1177/1082013219843395. View

17.

Guo K, Chen C . Investigation of Far-UVC (222 nm) disinfection of bioaerosols deposited on surfaces with different material properties. J Hazard Mater. 2023; 465:133358. DOI: 10.1016/j.jhazmat.2023.133358. View

18.

Bottichio L, Keaton A, Thomas D, Fulton T, Tiffany A, Frick A . Shiga Toxin-Producing Escherichia coli Infections Associated With Romaine Lettuce-United States, 2018. Clin Infect Dis. 2019; 71(8):e323-e330. PMC: 10982825. DOI: 10.1093/cid/ciz1182. View

19.

Raeiszadeh M, Adeli B . A Critical Review on Ultraviolet Disinfection Systems against COVID-19 Outbreak: Applicability, Validation, and Safety Considerations. ACS Photonics. 2023; 7(11):2941-2951. DOI: 10.1021/acsphotonics.0c01245. View

20.

Fransisca L, Feng H . Effect of surface roughness on inactivation of Escherichia coli O157:H7 87-23 by new organic acid-surfactant combinations on alfalfa, broccoli, and radish seeds. J Food Prot. 2012; 75(2):261-9. DOI: 10.4315/0362-028X.JFP-11-279. View