Characterization, Antibiotic Susceptibility, and Clonal Analysis of Carbapenem-Resistant Klebsiella Pneumoniae From Different Clinical Cases

Overview

Journal Cureus

Specialty Biomedical Engineering

Date 2024 Nov 20

PMID 39564009

Authors

Zanan M Taha

Affiliations

Soon will be listed here.

Abstract

Introduction: Carbapenem-resistant () is recognized for its great ability to resist prescription drugs and its association with severe infections in humans.

Objectives: This study was designed to evaluate the characteristic resistance spectrum, to characterize the implicated carbapenem-resistant genes (CRGs), and to determine the extent of genetic diversity among isolates from human clinical cases in Duhok province. Methodology: The VITEK-2 system was used to investigate the phenotypic antibiotic susceptibility of 23 isolated from distinct human clinical situations, multiplex PCR was used to assign the key common carbapenem-resistant genes (, -like, , and ) in phenotypically carbapenem-resistant isolates, and the Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) assay was utilized to ascertain the clonal associations among those isolates.

Results: Phenotypic resistance analysis revealed high resistance rates to various antibiotics, with all isolates exhibiting multidrug resistance (MDR). Coronavirus disease 2019 (COVID-19) patient isolates demonstrated significantly higher resistance compared to other sources. In addition, all isolates showed complete phenotypic resistance to carbapenems, PCR screening for CRGs identified blaOXA-48 as the predominant gene, present in all isolates. Genetic fingerprinting revealed diverse genotypes, with COVID-19 patient isolates exhibiting high similarity, contrasting with maximum diversity in non-COVID-19 clinical isolates.

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