Complex Immune Network and Regional Consistency in the Human Gastric Mucosa Revealed by High-resolution Spectral Cytometry

Overview

Journal Sci Rep

Specialty Science

Date 2024 Nov 20

PMID 39562636

Authors

Angel De Prado

Paloma Cal-Sabater

Aida Fiz-Lopez

Sandra Izquierdo

Daniel Corrales

Francisco Perez-Cozar

Juan H-Vazquez

Elisa Arribas-Rodriguez

Candido Perez-Segurado

Alvaro Martin Munoz

Jose A Garrote

Eduardo Arranz

Concepcion Maranon

Sara Cuesta-Sancho

Luis Fernandez-Salazar

David Bernardo

Affiliations

Soon will be listed here.

Abstract

The immune cellular landscape from the gastric mucosa remains largely unknown despite its relevance in several inflammatory conditions. Human gastric biopsies were obtained from the antrum, body and incisura from 10 individuals to obtain lamina propria mononuclear cells that were further characterized by spectral cytometry. Phenotypic hierarchical analyses identified a total of 52 different immune cell subsets within the human gastric mucosa revealing that T-cells (> 60%) and NK cells (> 20%) were the main populations. Within T-cells, CD4 and CD8 were equally represented with both subsets displaying mainly a memory and effector phenotype. NK cells, on the contrary, were largely of the early phenotype. No regional differences were observed for any subsets among the 3 locations. Following unsupervised analysis, a total of 82 clusters were found. Again, no differences were observed amongst locations although a great degree of inter-individual variability was found, largely influenced by the presence of H. pylori infection and dyspepsia. We have unraveled the human gastric immune cellular subset composition and a unique interindividual immune fingerprint with no inter-regional variations.

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