The Combination of Host-Associated R32 and Stachyose Promotes the Intestinal Health of Turbot (. L)

Overview

Journal Aquac Nutr

Publisher Wiley

Date 2024 Nov 18

PMID 39555546

Authors

Yaoyao Kong

Sifan Zhao

Weihao Ou

Kangsen Mai

Yanjiao Zhang

Affiliations

Soon will be listed here.

Abstract

An 8-week feeding trial was conducted to investigate the effects of host-associated R32 and stachyose on the intestinal mucosal defense system of turbot (. L). Three isonitrogenous and isolipidic diets were formulated: control diet (C), control diet with 1.0 × 10 CFU/g R32 (RC), and 1.0 × 10 CFU/g R32 + 1.5% stachyose (RS) supplementation separately. The results showed that diets RS and RC significantly inhibited the expression of cell development and apoptosis-related genes (, , , ); diets RS and RC significantly increased the expression of intestinal tight junction protein , while RS group significantly decreased the expression of ; diets RS and RC significantly decreased the expression of proinflammatory factors (, , ), diet RS also significantly decreased the expression of and , and increased the expression of . 16s rRNA gene sequencing results showed that diets RS and RC significantly decreased the abundance of conditional pathogenic bacteria (, , ). Among them, the abundance of in the RS group was the highest. It is concluded that the combination of stachyose and R32 had a more positive effect on intestinal cell development and barrier function and strengthened the intestinal mucosal defense system of turbot.

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