Poor Semen Quality is Associated with Impaired Antioxidant Response and Acute Phase Proteins and is Likely Mediated by High Cortisol Levels in Brucella-seropositive Dromedary Camel Bulls

Overview

Journal Sci Rep

Specialty Science

Date 2024 Nov 13

PMID 39537650

Authors

Ahmed Saad Ahmed Hassaneen

Anis Anis

Safaa Y Nour

Rasha Salah Mohamed

Islam M Wassif

Adel M El-Kattan

Hosny Ahmed Abdelgawad

Ragab H Mohamed

Affiliations

Soon will be listed here.

Abstract

Brucellosis in dromedary camel bulls leads to either temporary or permanent loss of fertility. Camel brucellosis is associated with both orchitis and epididymitis. However, the clinical signs of camel brucellosis are not clear as those in cattle. Therefore, this study aimed to diagnose camel brucellosis based on a serological screening using Rose Bengal plate test (RBPT) followed by competitive ELISA. To understand the impact of brucellosis on camel bull fertility, this study aimed to examine the semen characteristics, evaluate the testicular histopathology, examine hormonal profile, antioxidants and acute phase proteins (APP). A total of 150 mature bulls were used in this study. Blood samples were collected for serological, hormonal, and biochemical analysis. This study revealed that 6.6% and 7.3% of the examined bulls were Brucella-seropositive using RBPT and competitive ELISA, respectively. The Brucella-seropositive dromedary bulls showed poor semen quality, pathological changes orchitis, and lower testosterone. Moreover, our findings showed a higher cortisol level, and significant impairments in the measured APP and antioxidants in Brucella-seropositive bulls. In conclusion, the Brucella-seropositive dromedary bulls showed lower fertility due to poor semen quality and lower testosterone levels. Such lower fertility is likely mediated by high cortisol levels, and impaired APP and antioxidants' defense response.

References

Sharma R, Agarwal A . Role of reactive oxygen species in male infertility. Urology. 1996; 48(6):835-50. DOI: 10.1016/s0090-4295(96)00313-5. View

Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe S . Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014; 94(2):329-54. PMC: 4044300. DOI: 10.1152/physrev.00040.2012. View

Tibary A, El Allali K . Dromedary camel: A model of heat resistant livestock animal. Theriogenology. 2020; 154:203-211. DOI: 10.1016/j.theriogenology.2020.05.046. View

Menon A, Thundathil J, Wilde R, Kastelic J, Barkema H . Validating the assessment of bull sperm morphology by veterinary practitioners. Can Vet J. 2011; 52(4):407-8. PMC: 3058655. View

Shahin M, Khalil W, Saadeldin I, Swelum A, El-Harairy M . Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa. Animals (Basel). 2020; 10(1). PMC: 7022978. DOI: 10.3390/ani10010078. View

Sprague L, Al-Dahouk S, Neubauer H . A review on camel brucellosis: a zoonosis sustained by ignorance and indifference. Pathog Glob Health. 2012; 106(3):144-9. PMC: 4001573. DOI: 10.1179/2047773212Y.0000000020. View

El-Maaty A, Mohamed R, Hozyen H, El-Kattan A, Mahmoud M, H Ali A . Effect of exogenous progesterone treatment on ovarian steroid hormones and oxidant and antioxidant biomarkers during peak and low breeding seasons in dromedary she-camel. Vet World. 2019; 12(4):542-550. PMC: 6515829. DOI: 10.14202/vetworld.2019.542-550. View

Bay Karabulut A, Sonmez E, Bayindir Y . Effect of the treatment of brucellosis on leukocyte superoxide dismutase activity and plasma nitric oxide level. Ann Clin Biochem. 2005; 42(Pt 2):130-2. DOI: 10.1258/0004563053492801. View

Al-Qarawi A . Infertility in the dromedary bull: a review of causes, relations and implications. Anim Reprod Sci. 2005; 87(1-2):73-92. DOI: 10.1016/j.anireprosci.2004.11.003. View

10.

Mohamed R, Khalphallah A, Nakada K, Elmeligy E, Hassan D, Ebissy E . Clinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (). Vet Sci. 2021; 8(11). PMC: 8624123. DOI: 10.3390/vetsci8110247. View

11.

Al Eknah M . Reproduction in Old World camels. Anim Reprod Sci. 2000; 60-61:583-92. DOI: 10.1016/s0378-4320(00)00134-2. View

12.

Aitken J, Fisher H . Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioessays. 1994; 16(4):259-67. DOI: 10.1002/bies.950160409. View

13.

Alhussain H, Zughaier S, Gawish A, Mahmoud M, Yassine H, Thani A . Seroprevalence of camel brucellosis in Qatar. Trop Anim Health Prod. 2022; 54(6):351. PMC: 9581880. DOI: 10.1007/s11250-022-03335-z. View

14.

Eckersall P, Bell R . Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Vet J. 2010; 185(1):23-7. DOI: 10.1016/j.tvjl.2010.04.009. View

15.

El-Deeb W, Elmoslemany A . The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis. PeerJ. 2016; 4:e2161. PMC: 4957991. DOI: 10.7717/peerj.2161. View

16.

Ceciliani F, Giordano A, Spagnolo V . The systemic reaction during inflammation: the acute-phase proteins. Protein Pept Lett. 2002; 9(3):211-23. DOI: 10.2174/0929866023408779. View

17.

Petersen H, Nielsen J, Heegaard P . Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res. 2004; 35(2):163-87. DOI: 10.1051/vetres:2004002. View

18.

Lykkesfeldt J, Svendsen O . Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet J. 2006; 173(3):502-11. DOI: 10.1016/j.tvjl.2006.06.005. View

19.

Mohamed R, El-Maaty A, Abd El Hameed A, H Ali A . Impact of travel by walk and road on testicular hormones, oxidants, traces minerals, and acute phase response biomarkers of dromedary camels. Heliyon. 2021; 7(4):e06879. PMC: 8111580. DOI: 10.1016/j.heliyon.2021.e06879. View

20.

Matson K, Horrocks N, Versteegh M, Tieleman B . Baseline haptoglobin concentrations are repeatable and predictive of certain aspects of a subsequent experimentally-induced inflammatory response. Comp Biochem Physiol A Mol Integr Physiol. 2012; 162(1):7-15. DOI: 10.1016/j.cbpa.2012.01.010. View