Mechanically Tunable, Compostable, Healable and Scalable Engineered Living Materials

Overview

Journal Nat Commun

Specialty Biology

Date 2024 Nov 12

PMID 39532836

Authors

Avinash Manjula-Basavanna

Anna M Duraj-Thatte

Neel S Joshi

Affiliations

Soon will be listed here.

Abstract

Advanced design strategies are essential to realize the full potential of engineered living materials, including their biodegradability, manufacturability, sustainability, and ability to tailor functional properties. Toward these goals, we present mechanically engineered living material with compostability, healability, and scalability - a material that integrates these features in the form of a stretchable plastic that is simultaneously flushable, compostable, and exhibits the characteristics of paper. This plastic/paper-like material is produced in scalable quantities (0.5-1 g L), directly from cultured bacterial biomass (40%) containing engineered curli protein nanofibers. The elongation at break (1-160%) and Young's modulus (6-450 MPa) is tuned to more than two orders of magnitude. By genetically encoded covalent crosslinking of curli nanofibers, we increase the Young's modulus by two times. The designed engineered living materials biodegrade completely in 15-75 days, while its mechanical properties are comparable to petrochemical plastics and thus may find use as compostable materials for primary packaging.

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