MicroRNA319-mediated Gene Regulatory Network Impacts Leaf Development and Morphogenesis in Poplar

Overview

Journal For Res (Fayettev)

Date 2024 Nov 11

PMID 39524520

Authors

Yanxia Cheng

Lihu Wang

Manzar Abbas

Xiong Huang

Qiao Wang

Aimin Wu

Hairong Wei

Shaobing Peng

Xinren Dai

Quanzi Li

Affiliations

Soon will be listed here.

Abstract

MicroRNA319 (miR319) has been implicated in leaf development in a number of plant species. Here we study the roles of miR319a and its regulated network in leaf development in poplars. Over-expression of miR319a in × caused dwarf statures, narrow leaf blades and serrated leaf margins. The vascular bundles and bundle sheaths in transgenic leaves had more layers of cells than those in the leaves of control plants, indicating enhanced lignification in these cells. Among the 93 putative targets of miR319a predicted with the psRNATarget tool, only three genes, (, , and ), were differentially expressed in the leaves of -over-expression transgenic lines. With the RNA-seq data sets from multiple over-expression transgenic lines, we built a three-layered gene regulatory network mediated by miR319a using Top-down graphic Gaussian model (GGM) algorithm that is capable of capturing causal relationships from transcriptomic data. The results support that primarily regulates the lignin biosynthesis, leaf development and differentiation as well as photosynthesis via , and regulatory modules.

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