Frequencies or Absolute Numbers? Cluster Analysis of Frequencies and Absolute Numbers of B-Cell Subsets in Dialysis Patients Who Are Candidates for Kidney Transplantation Reveals Different Profiles

Overview

Journal J Clin Med

Specialty General Medicine

Date 2024 Nov 9

PMID 39518592

Authors

Ariadni Fouza

Asimina Fylaktou

Anneta Tagkouta

Maria Daoudaki

Lampros Vagiotas

Efstratios Kasimatis

Aliki Xochelli

Vasilki Nikolaidou

Georgios Katsanos

Georgios Tsoulfas

Lemonia Skoura

Aikaterini Papagianni

Nikolaos Antoniadis

Affiliations

Soon will be listed here.

Abstract

Detailed characterization of B cells in dialysis patients who are candidates for kidney transplant is still lacking, with little information on how dialysis duration and modality impact B cell subsets. Cluster analysis of flow cytometry determined the frequencies and absolute numbers of B-cell subsets and divided the cohort of 78 candidates into two distinct clusters, one with shorter and one with longer dialysis duration. The immune profiles of the clusters differed depending on whether frequencies or absolute counts were considered. In long-term dialysis patients, the frequency of total memory, double negative and marginal zone B cells increased, while the frequency of naive and regulatory B cells decreased. This pattern was reversed in short-term dialysis patients, with a decrease in memory and an increase in naive and regulatory populations. The B subset number decreased significantly in long-term dialysis patients, while it increased significantly in short-term dialysis patients. The dialysis modality affected the frequency-based subset immune profiles. It is important to determine whether the evaluation is based on frequencies or absolute numbers. The different distribution of B cell subsets in the clusters, in terms of frequencies and absolute numbers, was influenced by dialysis duration. Modality and age only influenced the frequencies.

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