Polarization Modulated Spectroscopic Ellipsometry-based Surface Plasmon Resonance Biosensor for E. Coli K12 Detection

Overview

Journal Sci Rep

Specialty Science

Date 2024 Nov 7

PMID 39511384

Authors

Soraya Zangenehzadeh

Emil Agocs

Fenja Schroder

Nassima Amroun

Rebekka Biedendieck

Dieter Jahn

Axel Gunther

Lei Zheng

Bernhard Roth

Hans-Hermann Johannes

Wolfgang Kowalsky

Affiliations

Soon will be listed here.

Abstract

In this work, we report on the application of the polarization modulated spectroscopic ellipsometry-based surface plasmon resonance method for sensitive detection of microorganisms in Kretschmann configuration. So far, rotating analyzer and single wavelength polarization modulation methods have widely been investigated for phase sensitive surface plasmon resonance measurement. In this study, a much simpler optical setup relying on fast electro-optic phase modulator crystals is introduced for bacteria detection. A beta barium borate crystal connected to a function generator is adapted for generating phase shifts in the millisecond regime to extract the ellipsometric angles ( and ) under the surface plasmon resonance condition. For detection, the gold surface was functionalized with anti-Escherichia coli antibodies, and E. coli K12 was attached to them. We show that polarization modulated spectroscopic ellipsometry achieves a refractive index resolution in the order of RIU, and a limit of detection of CFU/mL for E. coli K12 which is compatible with other surface plasmon resonance based phase sensitive methods with more complex detection concepts. As a follow-up step, an optical model can be developed to enhance this biosensor's performance, and applications for sorting and detecting other biological targets will be investigated.

References

Zhou Y, Zhou Z, Zheng L, Gong Z, Li Y, Jin Y . Urinary Tract Infections Caused by Uropathogenic : Mechanisms of Infection and Treatment Options. Int J Mol Sci. 2023; 24(13). PMC: 10341809. DOI: 10.3390/ijms241310537. View

Markowicz P, Law W, Baev A, Prasad P, Patskovsky S, Kabashin A . Phase-sensitive time-modulated surface plasmon resonance polarimetry for wide dynamic range biosensing. Opt Express. 2009; 15(4):1745-54. DOI: 10.1364/oe.15.001745. View

Allocati N, Masulli M, Alexeyev M, Di Ilio C . Escherichia coli in Europe: an overview. Int J Environ Res Public Health. 2013; 10(12):6235-54. PMC: 3881111. DOI: 10.3390/ijerph10126235. View

Mendes Silva D, Domingues L . On the track for an efficient detection of Escherichia coli in water: A review on PCR-based methods. Ecotoxicol Environ Saf. 2014; 113:400-11. DOI: 10.1016/j.ecoenv.2014.12.015. View

Pang B, Zhao C, Li L, Song X, Xu K, Wang J . Development of a low-cost paper-based ELISA method for rapid Escherichia coli O157:H7 detection. Anal Biochem. 2017; 542:58-62. DOI: 10.1016/j.ab.2017.11.010. View

Shen S, Liu T, Guo J . Optical phase-shift detection of surface plasmon resonance. Appl Opt. 2008; 37(10):1747-51. DOI: 10.1364/ao.37.001747. View

Homola J . Surface plasmon resonance sensors for detection of chemical and biological species. Chem Rev. 2008; 108(2):462-93. DOI: 10.1021/cr068107d. View

Fode-Vaughan K, Maki J, Benson J, Collins M . Direct PCR detection of Escherichia coli O157:H7. Lett Appl Microbiol. 2003; 37(3):239-43. DOI: 10.1046/j.1472-765x.2003.01386.x. View

Nataro J, Kaper J . Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998; 11(1):142-201. PMC: 121379. DOI: 10.1128/CMR.11.1.142. View

10.

Wang Y, Ye Z, Si C, Ying Y . Subtractive inhibition assay for the detection of E. coli O157:H7 using surface plasmon resonance. Sensors (Basel). 2011; 11(3):2728-39. PMC: 3231628. DOI: 10.3390/s110302728. View

11.

Ghayoor R, Zangenehzadeh S, Keshavarz A . Design of High-Sensitivity Surface Plasmon Resonance Sensor Based on Nanostructured Thin Films for Effective Detection of DNA Hybridization. Plasmonics. 2022; 17(4):1831-1841. PMC: 9198207. DOI: 10.1007/s11468-022-01669-w. View

12.

Liu H, Liu W, Jin G . Detection of Exosomes Using Total Internal Reflected Imaging Ellipsometry. Biosensors (Basel). 2021; 11(5). PMC: 8160712. DOI: 10.3390/bios11050164. View

13.

Wu L, Chu H, Koh W, Li E . Highly sensitive graphene biosensors based on surface plasmon resonance. Opt Express. 2010; 18(14):14395-400. DOI: 10.1364/OE.18.014395. View

14.

Arya S, Singh A, Naidoo R, Wu P, McDermott M, Evoy S . Chemically immobilized T4-bacteriophage for specific Escherichia coli detection using surface plasmon resonance. Analyst. 2010; 136(3):486-92. DOI: 10.1039/c0an00697a. View

15.

Shih C, Chang C, Hsu M, Lin J, Kuan C, Wang H . Paper-based ELISA to rapidly detect Escherichia coli. Talanta. 2015; 145:2-5. DOI: 10.1016/j.talanta.2015.07.051. View

16.

Damborsky P, Svitel J, Katrlik J . Optical biosensors. Essays Biochem. 2016; 60(1):91-100. PMC: 4986466. DOI: 10.1042/EBC20150010. View

17.

Tawil N, Sacher E, Mandeville R, Meunier M . Surface plasmon resonance detection of E. coli and methicillin-resistant S. aureus using bacteriophages. Biosens Bioelectron. 2012; 37(1):24-9. DOI: 10.1016/j.bios.2012.04.048. View

18.

Sinibaldi A, Anopchenko A, Rizzo R, Danz N, Munzert P, Rivolo P . Angularly resolved ellipsometric optical biosensing by means of Bloch surface waves. Anal Bioanal Chem. 2015; 407(14):3965-74. DOI: 10.1007/s00216-015-8591-8. View

19.

Wang S, Xie J, Jiang M, Chang K, Chen R, Ma L . The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7. Sensors (Basel). 2016; 16(11). PMC: 5134515. DOI: 10.3390/s16111856. View

20.

Chen C, Wang J . Optical biosensors: an exhaustive and comprehensive review. Analyst. 2020; 145(5):1605-1628. DOI: 10.1039/c9an01998g. View