Effects of Dietary Protease Supplementation on Soybean Meal Protein, Dry Matter Digestibility, and Productive Performance in Starter-to-finisher Pigs

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2024 Nov 7

PMID 39507778

Authors

Phubet Satsook

Surapan Jitviriyanon

Anchalee Khongpradit

Sirinapa Chungopast

Chanwit Kaewtapee

Nitipong Homwong

Affiliations

Soon will be listed here.

Abstract

Background And Aim: Pig industries are currently facing a crisis in terms of protein and energy costs. Proteases were used to increase protein digestibility and metabolizable energy (ME) in diets. This study evaluated the effects of protease supplementation on protein digestibility and productive performance in starter-to-finisher pigs.

Materials And Methods: A total of 691 starter pigs were randomly allocated into three dietary treatments using a randomized complete block design. Diets were provided in three phases according to body weight (BW): Starter, grower, and finisher phases. Each phase was fed for 30, 60, and 24 days of treatment diets as T1: basal diet and T2 and T3: the basal diet supplemented with 240 ppm protease reduced by 50 kcal/kg ME plus 1% crude protein (CP) and by 100 kcal/kg ME plus 2% CP, respectively. Protease and protein digestibility were measured. BW and feed intake were recorded to calculate the average daily gain (ADG), average daily feed intake (ADFI), feed-to-gain (F:G), and gain-to-feed (G:F) ratios.

Results: There were no significant differences (p > 0.05) in the percentage of protein digestibility between the groups with and without protease supplementation. In the finisher phase, T2 had lower (p < 0.05) ADFI and F:G than T1 and T3. Overall, T3 had lower (p < 0.05) ADG, ADFI, and F:G than T1 and T2.

Conclusion: Protease supplementation significantly affects protein digestibility. Supplementing basal diets with 240 ppm protease reduced ME by 50 kcal/kg and CP by 1% without affecting ADG, ADFI, F:G, and G:F ratios for starter-to-finisher pigs.

References

Kim Y, Lee J, Kim T, Song M, Yun W, Oh H . Effect of low protein diets added with protease on growth performance, nutrient digestibility of weaned piglets and growing-finishing pigs. J Anim Sci Technol. 2021; 63(3):491-500. PMC: 8204005. DOI: 10.5187/jast.2021.e49. View

Wang D, Zeng Z, Piao X, Li P, Xue L, Zhang Q . Effects of keratinase supplementation of corn-soybean meal based diets on apparent ileal amino acid digestibility in growing pigs and serum amino acids, cytokines, immunoglobulin levels and loin muscle area in nursery pigs. Arch Anim Nutr. 2011; 65(4):290-302. DOI: 10.1080/1745039x.2011.586140. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Duarte M, Zhou F, Dutra Jr W, Kim S . Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs. Anim Nutr. 2020; 5(4):351-358. PMC: 6920460. DOI: 10.1016/j.aninu.2019.04.005. View

Park S, Lee J, Cowieson A, Pappenberger G, Woyengo T . Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets. J Anim Sci. 2020; 98(10). PMC: 7568435. DOI: 10.1093/jas/skaa308. View

Lee J, Choe J, Kang J, Cho J, Park S, Perez-Maldonado R . Dietary protease improves growth rate and protein digestibility of growing-finishing pigs. J Anim Sci Technol. 2020; 62(3):313-320. PMC: 7288229. DOI: 10.5187/jast.2020.62.3.313. View

Shahir M, Rahimi R, Taheri H, Heidariniya A, Baradaran N, Asadi Kermani Z . Effect of protein source and protease addition on performance, blood metabolites and nutrient digestibility of turkeys fed on low-protein diets from 28 to 55 d post hatch. Br Poult Sci. 2016; 57(3):390-6. DOI: 10.1080/00071668.2016.1172202. View

Park S, Lee J, Yang B, Cho J, Kim S, Kang J . Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs. J Anim Sci Technol. 2020; 62(1):21-30. PMC: 7008117. DOI: 10.5187/jast.2020.62.1.21. View

Cowieson A, Toghyani M, Kheravii S, Wu S, Romero L, Choct M . A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn/wheat-based diets supplemented with.... Poult Sci. 2018; 98(3):1321-1332. PMC: 6377439. DOI: 10.3382/ps/pey456. View

10.

Chen H, Zhang S, Park I, Kim S . Impacts of energy feeds and supplemental protease on growth performance, nutrient digestibility, and gut health of pigs from 18 to 45 kg body weight. Anim Nutr. 2018; 3(4):359-365. PMC: 5941274. DOI: 10.1016/j.aninu.2017.09.005. View

11.

Fuller M, Reeds P . Nitrogen cycling in the gut. Annu Rev Nutr. 1998; 18:385-411. DOI: 10.1146/annurev.nutr.18.1.385. View

12.

Zuo J, Ling B, Long L, Li T, Lahaye L, Yang C . Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets. Anim Nutr. 2018; 1(4):276-282. PMC: 5940980. DOI: 10.1016/j.aninu.2015.10.003. View

13.

Cowieson A, Bhuiyan M, Sorbara J, Pappenberger G, Pedersen M, Choct M . Contribution of individual broilers to variation in amino acid digestibility in soybean meal and the efficacy of an exogenous monocomponent protease. Poult Sci. 2020; 99(2):1075-1083. PMC: 7587858. DOI: 10.1016/j.psj.2019.10.001. View

14.

Gupta R, Rajput R, Sharma R, Gupta N . Biotechnological applications and prospective market of microbial keratinases. Appl Microbiol Biotechnol. 2013; 97(23):9931-40. DOI: 10.1007/s00253-013-5292-0. View

15.

Zhu Q, Wang Y, Liu Y, Yu B, He J, Zheng P . Effects of a Novel Protease on Growth Performance, Nutrient Digestibility and Intestinal Health in Weaned Piglets. Animals (Basel). 2022; 12(20). PMC: 9597718. DOI: 10.3390/ani12202803. View