The Sodium/ascorbic Acid Co-transporter SVCT2 Distributes in a Striated Membrane-enriched Domain at the M-band Level in Slow-twitch Skeletal Muscle Fibers

Overview

Journal Biol Res

Specialty Biology

Date 2024 Nov 7

PMID 39506870

Authors

Daniel Sandoval

Jessica Mella

Jorge Ojeda

Francisca Bermedo-Garcia

Marcela Low

Sylvain Marcellini

Maite A Castro

Mariana Casas

Enrique Jaimovich

Juan Pablo Henriquez

Affiliations

Soon will be listed here.

Abstract

Background: Vitamin C plays key roles in cellular homeostasis, functioning as a potent antioxidant and a positive regulator of cell differentiation. In skeletal muscle, the vitamin C/sodium co-transporter SVCT2 is preferentially expressed in oxidative slow fibers. SVCT2 is up-regulated during the early fusion of primary myoblasts and decreases during initial myotube growth, indicating the relevance of vitamin C uptake via SVCT2 for early skeletal muscle differentiation and fiber-type definition. However, our understanding of SVCT2 expression and function in adult skeletal muscles is still limited.

Results: In this study, we demonstrate that SVCT2 exhibits an intracellular distribution in chicken slow skeletal muscles, following a highly organized striated pattern. A similar distribution was observed in human muscle samples, chicken cultured myotubes, and isolated mouse myofibers. Immunohistochemical analyses, combined with biochemical cell fractionation experiments, reveal a strong co-localization of SVCT2 with intracellular detergent-soluble membrane fractions at the central sarcomeric M-band, where it co-solubilizes with sarcoplasmic reticulum proteins. Remarkably, electrical stimulation of cultured myofibers induces the redistribution of SVCT2 into a vesicular pattern.

Conclusions: Our results provide novel insights into the dynamic roles of SVCT2 in different intracellular compartments in response to functional demands.

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