Inhibition of the Notch Signal Transducer CSL by Pkc53E-mediated Phosphorylation to Fend off Parasitic Immune Challenge in

Overview

Journal Elife

Specialty Biology

Date 2024 Nov 6

PMID 39503739

Authors

Sebastian Deichsel

Lisa Frankenreiter

Johannes Fechner

Bernd M Gahr

Mirjam Zimmermann

Helena Mastel

Irina Preis

Anette Preiss

Anja C Nagel

Affiliations

Soon will be listed here.

Abstract

Notch signalling activity regulates hematopoiesis in and vertebrates alike. Parasitoid wasp infestation of larvae, however, requires a timely downregulation of Notch activity to allow the formation of encapsulation-active blood cells. Here, we show that the CSL transcription factor Suppressor of Hairless [Su(H)] is phosphorylated at Serine 269 in response to parasitoid wasp infestation. As this phosphorylation interferes with the DNA binding of Su(H), it reversibly precludes its activity. Accordingly, phospho-deficient mutants are immune-compromised. A screen for kinases involved in Su(H) phosphorylation identified Pkc53E, required for normal hematopoiesis as well as for parasitoid immune response. Genetic and molecular interactions support the specificity of the Su(H)-Pkc53E relationship. Moreover, phorbol ester treatment inhibits Su(H) activity in vivo and in human cell culture. We conclude that Pkc53E targets Su(H) during parasitic wasp infestation, thereby remodelling the blood cell population required for wasp egg encapsulation.

Citing Articles

Numerous Serine/Threonine Kinases Affect Blood Cell Homeostasis in .

Deichsel S, Gahr B, Mastel H, Preiss A, Nagel A Cells. 2024; 13(7.

PMID: 38607015 PMC: 11011202. DOI: 10.3390/cells13070576.

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