A Clinical Case of B-cell Lymphoma in a Japanese Black Cow with Monoclonal Proliferation of B-cells As Revealed by Polymerase Chain Reaction Based on the Immunoglobulin Light Chain Gene

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 2024 Nov 4

PMID 39496453

Authors

Tatsuki Nagata

Toshiyuki Harada

Masaki Maezawa

James K Chambers

Kazuyuki Uchida

Hisashi Inokuma

Affiliations

Soon will be listed here.

Abstract

A 35-month-old Japanese Black cow was presented to a local veterinarian with a main complaint of premature labor. There was no swelling of lymph nodes on the body surface and no palpable mass on rectal examination. Although atypical lymphocytes were observed in the peripheral blood, no lymphocytosis was recorded. On Day 12, the cow developed hindlimb paresis and was euthanized. Necropsy revealed swelling of some lymph nodes, and white nodules in uterus and right atrial appendage. Histopathological examination revealed B-cell lymphoma. In clonality analysis of peripheral blood and tumor tissue, monoclonal proliferation was detected by PCR for immunoglobulin light chain (IgL) λ, suggesting neoplastic proliferation of B-cell. IgL-PCR is thought to be a possible tool for diagnosis of B-cell lymphoma.

References

Ferrer J . Bovine lymphosarcoma. Adv Vet Sci Comp Med. 1980; 24:1-68. View

van Dongen J, Langerak A, Bruggemann M, Evans P, Hummel M, Lavender F . Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. 2003; 17(12):2257-317. DOI: 10.1038/sj.leu.2403202. View

Nagata T, Oouchi Y, Yamamoto K, Maezawa M, Chambers J, Uchida K . A clinical case of lymphoma with hindlimb paresis due to mass formation in the spinal canal in a Japanese Black cow. J Vet Med Sci. 2024; 86(9):969-973. PMC: 11422685. DOI: 10.1292/jvms.24-0152. View

Maezawa M, Watanabe K, Horiuchi N, Matsumoto K, Kobayashi Y, Inokuma H . Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene. J Vet Med Sci. 2019; 82(1):61-63. PMC: 6983668. DOI: 10.1292/jvms.19-0418. View

Daiji Y, Maezawa M, Ishikawa K, Chambers J, Uchida K, Inokuma H . Enzootic bovine leukosis caused by bovine leukemia virus classified as Group C based on viral whole genome sequencing in a 23-month-old Holstein-Friesian heifer. J Vet Med Sci. 2023; 85(12):1291-1295. PMC: 10788170. DOI: 10.1292/jvms.23-0354. View

Akagami M, Oya S, Kashima Y, Seki S, Ouchi Y, Hayama Y . A hematologic key for bovine leukemia virus screening in Japanese black cattle. J Vet Diagn Invest. 2019; 31(4):568-571. PMC: 6857024. DOI: 10.1177/1040638719848449. View

Sakamoto L, Ohbayashi T, Matsumoto K, Kobayashi Y, Inokuma H . Serum thymidine kinase activity as a useful marker for bovine leukosis. J Vet Diagn Invest. 2009; 21(6):871-4. DOI: 10.1177/104063870902100619. View

Ishihara K, Ohtani T, Kitagawa H, Onuma M . Clinical studies on bovine leukemia in Japanese black cattle. III. Serum lactate dehydrogenase activity and its isoenzyme pattern in groups of leukemic cattle and those negative or positive for antibody against bovine leukemia virus. Nihon Juigaku Zasshi. 1980; 42(3):289-95. DOI: 10.1292/jvms1939.42.289. View

Keller S, Vernau W, Moore P . Clonality Testing in Veterinary Medicine: A Review With Diagnostic Guidelines. Vet Pathol. 2016; 53(4):711-25. DOI: 10.1177/0300985815626576. View

10.

Ghorbian S, Jahanzad I, Javadi G, Sakhinia E . Evaluation diagnostic usefulness of immunoglobulin light chains (Igκ, Igλ) and incomplete IGH D-J clonal gene rearrangements in patients with B-cell non-Hodgkin lymphomas using BIOMED-2 protocol. Clin Transl Oncol. 2014; 16(11):1006-11. DOI: 10.1007/s12094-014-1188-4. View

11.

Hwang M, Darzentas N, Bienzle D, Moore P, Guscetti F, Morrison J . A review of canine B cell clonality assays and primer set optimization using large-scale repertoire data. Vet Immunol Immunopathol. 2019; 209:45-52. DOI: 10.1016/j.vetimm.2019.01.002. View

12.

Burton A, Nydam D, Long E, Divers T . Signalment and clinical complaints initiating hospital admission, methods of diagnosis, and pathological findings associated with bovine lymphosarcoma (112 cases). J Vet Intern Med. 2010; 24(4):960-4. DOI: 10.1111/j.1939-1676.2010.0537.x. View

13.

Amara K, Trimeche M, Ziadi S, Sriha B, Mokni M, Korbi S . PCR-based clonality analysis of B-cell lymphomas in paraffin-embedded tissues: diagnostic value of immunoglobulin kappa and lambda light chain gene rearrangement investigation. Pathol Res Pract. 2006; 202(6):425-31. DOI: 10.1016/j.prp.2005.09.005. View

14.

Rout E, Burnett R, Yoshimoto J, Avery P, Avery A . Assessment of immunoglobulin heavy chain, immunoglobulin light chain, and T-cell receptor clonality testing in the diagnosis of feline lymphoid neoplasia. Vet Clin Pathol. 2019; 48 Suppl 1:45-58. DOI: 10.1111/vcp.12767. View